Development of hepatorenal syndrome in bile duct ligated rats

• Published in: World journal of gastroenterology : WJG Vol. 14; no. 28; pp. 4505 - 4511
• Main Authors: Pereira, Regina M, dos Santos, Robson A S, Oliveira, Eduardo A, Leite, Virginia H R, Dias, Filipi L C, Rezende, Alysson S, Costa, Lincoln P, Barcelos, Luciola S, Teixeira, Mauro M, Simoes e Silva, Ana Cristina
• Format: Journal Article
• Language: English
• Published: United States Departamento de Ciências Biologicas, Centre Universitário de Belo Horizonte-UNIBH, Av. Mário Wemeck, Belo Horizonte, Minas Gerais 30150-281, Brazil%Laboratório de Hipertensao, Departamento de Fisiologia e Biofisica, Institute de Ciências Biológicas (ICB), Universidade Federal de Minas Gerais (UFMG), Av. Antonio Carlos, 6627, Belo Horizonte, Minas Gerais 30150-281, Brazil%Departamento de Pediatria, Faculdade de Medicina, UFMG, Av. Alfredo Balena 190, Belo Horizonte, Minas Gerais 30150-281, Brazil%Laboratório de Imunofarmacologia, Departamento de Bioquimica e Imunologia, ICB, UFMG, Av. Antonio Carlos 6627, Belo Horizonte, Minas Gerais 30150-281, Brazil 28.07.2008; The WJG Press and Baishideng
• Subjects: Animals; Bile Ducts - surgery; Creatinine - blood; Disease Models, Animal; Disease Progression; Hepatorenal Syndrome - etiology; Hepatorenal Syndrome - metabolism; Hepatorenal Syndrome - physiopathology; Hydroxyproline - metabolism; Kidney - metabolism; Kidney - pathology; Kidney - physiopathology; Ligation; Liver - metabolism; Liver - pathology; Liver - physiopathology; Male; Rapid Communication; Rats; Rats, Wistar; 肾功能; 胆管结扎; 高血压蛋白原酶; Renin angiotensin system; Renal function; Hepatorenal syndrome; Bile duct ligation
• ISSN: 1007-9327; 2219-2840
• DOI: 10.3748/wjg.14.4505

AIM： To evaluate in bile duct ligated rats whether there were progressive alterations of renal function without changes in histopathology. METHODS： Male Wistar rats were submitted to sham-surgery or bile duct ligation （BDL） and divided according to the post-procedure time （2, 4 and 6-wk）. To determine renal function parameters, rats were placed in metabolic cages and, at the end of the experiment, blood and urine samples were obtained. Histology and hydroxyproline content were analyzed in liver and renal tissue. RESULTS： Rats with 2 wk of BDL increased free water clearance （P = 0.02）, reduced urinary osmolality （P = 0.03） and serum creatinine （P = 0.01） in comparison to the sham group. In contrast, rats at 6 wk of BDL showed features of HRS, including significant increase in serum creatinine and reductions in creatinine clearance, water excretion and urinary sodium concentration. Rats with 4 wk of BDL exhibited an intermediate stage of renal dysfunction. Progressive hepatic fibrosis according to post-procedure time was confirmed by histology. The increased levels of liver hydroxyproline contrasted with the absence of structural changes in the kidney, as assessed by histology and unchanged hydroxyproline content in renal tissue. CONCLUSION： Our data show that BDL produced progressive renal dysfunction without structural changes in the kidney, characterizing HRS. The present model will be useful to understand the pathophysiology of HRS.