Genetic variability of Blastocystis sp. isolates obtained from cancer and HIV/AIDS patients

• Published in: Parasitology research (1987) Vol. 105; no. 5; pp. 1283 - 1286
• Main Authors: Tan, T. C., Ong, S. C., Suresh, K. G.
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer-Verlag 01.10.2009; Springer
• Subjects: acquired immunodeficiency syndrome; Animals; Biological and medical sciences; Biomedical and Life Sciences; Biomedicine; Blastocystis; Blastocystis - classification; Blastocystis - genetics; Blastocystis - isolation & purification; Blastocystis Infections; Blastocystis Infections - parasitology; classification; complications; DNA Primers; DNA Primers - genetics; DNA, Protozoan; DNA, Protozoan - genetics; Fundamental and applied biological sciences. Psychology; General aspects; General aspects and techniques. Study of several systematic groups. Models; genetic variation; genetics; Genotype; HIV Infections; HIV Infections - complications; Human immunodeficiency virus; Humans; Immunology; Invertebrates; isolation & purification; Medical Microbiology; methods; Microbiology; Neoplasms; Neoplasms - complications; Original Paper; parasitology; patients; polymerase chain reaction; Polymerase Chain Reaction - methods; Polymorphism, Genetic; Protozoa; Blastocystis Infection; Diarrheal Illness; Immunocompromised Individual; Acute Urticaria; Germani; Infection; Virus; Protozoa; Lobosea; Genetic variability; Viral disease; Retroviridae; Parasite; AIDS; Human immunodeficiency virus; Lentivirus
• ISSN: 0932-0113; 1432-1955
• DOI: 10.1007/s00436-009-1551-5

This represents the first study to determine the genetic diversity of Blastocystis sp. among cancer and HIV/AIDS patients. Forty Blastocystis sp. isolates obtained from 20 cancer and 20 HIV/AIDS patients were genotyped by PCR using seven pairs of known sequenced-tagged site primers. Out of the 40 isolates, 38 were identified as one of the known genotypes and two isolates were negative with all the STS primers. Blastocystis sp. subtype 3 which is reported to be associated with disease was found to be predominant among the study subjects.