Mutational Analysis of the Bovine Respiratory Syncytial Virus Nucleocapsid Protein Using a Minigenome System: Mutations That Affect Encapsidation, RNA Synthesis, and Interaction with the Phosphoprotein

• Published in: Virology (New York, N.Y.) Vol. 270; no. 1; pp. 215 - 228
• Main Authors: Khattar, Sunil K., Yunus, Abdul S., Collins, Peter L., Samal, Siba K.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 25.04.2000
• Subjects: animal diseases; animal health; Animals; Binding Sites; Bovine respiratory syncytial virus; BRSV-CAT minigenome replication system; Cattle; Cell Line; Cysteine - genetics; Cysteine - metabolism; Genes, Reporter - genetics; Genome, Viral; HN Protein; Humans; Micrococcal Nuclease - metabolism; Mutation - genetics; Nucleocapsid - chemistry; Nucleocapsid - genetics; Nucleocapsid - metabolism; nucleocapsid protein; phosphoprotein P; Phosphoproteins - metabolism; Respiratory Syncytial Virus, Bovine - genetics; Respiratory Syncytial Virus, Bovine - growth & development; Respiratory Syncytial Virus, Bovine - metabolism; RNA, Antisense - biosynthesis; RNA, Antisense - genetics; RNA, Antisense - metabolism; RNA, Viral - biosynthesis; RNA, Viral - genetics; RNA, Viral - metabolism; Sequence Deletion - genetics; Templates, Genetic; Transcription, Genetic - genetics; Transfection; viral diseases of animals and humans; Viral Envelope Proteins; Viral Proteins - metabolism; Virus Assembly - genetics

The nucleocapsid (N) protein of bovine respiratory syncytial virus (BRSV) is a multifunctional protein that plays a central role in transcription and replication of viral genomic RNA. To investigate the domains and specific residues involved in different N activities, we generated a total of 27 dele...