Hepatic injury induced by carbon dioxide pneumoperitoneum in experimental rats

• Published in: World journal of gastroenterology : WJG Vol. 15; no. 24; pp. 3060 - 3064
• Main Authors: Xu, Gui-Sen, Liu, He-Nian, Li, Jun, Wu, Xiao-Ling, Dai, Xue-Mei, Liu, Ying-Hai
• Format: Journal Article
• Language: English
• Published: United States Department of Anesthesia, General Hospital of Chengdu Military Command Area, Chengdu 610083, Sichuan Province,China%Department of Digestion, General Hospital of Chengdu Military Command Area, Chengdu 610083, Sichuan Province, China 28.06.2009; The WJG Press
• Subjects: Alanine Transaminase - metabolism; Animals; Aspartate Aminotransferases - metabolism; Brief; Carbon Dioxide - toxicity; Hypoxia-Inducible Factor 1, alpha Subunit - genetics; Hypoxia-Inducible Factor 1, alpha Subunit - metabolism; Laparoscopy - adverse effects; Liver - injuries; Liver - metabolism; Liver - physiopathology; Male; Malondialdehyde - metabolism; Mitochondria - metabolism; Pneumoperitoneum, Artificial - adverse effects; Random Allocation; Rats; Rats, Sprague-Dawley; RNA, Messenger - metabolism; Superoxide Dismutase - metabolism; 二氧化碳; 肝损伤; Carbon dioxide pneumoperitoneum; Anoxia; Hepatic injury; Rat; Laparoscopic operation
• ISSN: 1007-9327; 2219-2840
• DOI: 10.3748/wjg.15.3060

AIM： To observe the hepatic injury induced by carbon dioxide pneumoperitoneum in rats and to explore its potential mechanism. METHODS： Thirty healthy male SD rats were randomly divided into control group （n = 10）, 0 h experimental group （n = 10） and 1 h experimental group （n = 10） after sham operation with carbon dioxide pneumoperitoneum. Histological changes in liver tissue were observed with hematoxylineosin staining. Liver function was assayed with an automatic biochemical analyzer. Concentration of malonyldialdehyde （MDA） and activity of superoxide dismutase （SOD） were assayed by colorimetry. Activity of adenine nucleotide translocator in liver tissue was detected with the atractyloside-inhibitor stop technique. Expression of hypoxia inducible factor-1 （HIF-1） mRNA in liver tissue was detected with in situ hybridization. RESULTS： Carbon dioxide 60 min could induce liver pneumoperitoneum for injury in rats. Alanine aminotransferase and aspartate aminotransferase were 95.7 ± 7.8 U/L and 86.8 ± 6.9 U/L in 0 h experimental group, and 101.4 ± 9.3 U/L and 106.6 ±8.7 U/L in 1 h experimental group. However, no significant difference was found in total billirubin, albumin, and pre-albumin in the three groups. In 0 h experimental group, the concentration of MDA was 9.83 ±2.53 μmol/g in liver homogenate and 7.64 ± 2.19 μmol/g in serum respectively, the activity of SOD was 67.58±9.75 nu/mg in liver and 64.47 ± 10.23 nu/mg in serum respectively. In 1 h experimental group, the concentration of MDA was 16.57±3.45 μmol/g in liver tissue and 12.49 ±4.21 μmol/g in serum respectively, the activity of SOD was 54.29 ±7.96 nu/mg in liver tissue and 56.31 ±9.85 nu/mg in serum respectively. The activity of ANT in liver tissue was 9.52 ± 1.56 in control group, 6.37± 1.33 in 0 h experimental group and 7.2 8±1.45 （10^-9 mol/min per gram protein） in 1 h experimental group, respectively. The expression of HIF-1 mRNA in liver tissue was not detected in control group, and its optical density difference value was 6.14±1.03 in 0 h experimental group and 9.51 ± 1.74 in 1 h experimental group, respectively. CONCLUSION： Carbon dioxide pneumoperitoneum during the sham operation can induce hepatic injury in rats. The probable mechanisms of liver injury include anoxia, ischemia reperfusion and oxidative stress. Liver injury should be avoided during clinical laparoscopic operation with carbon dioxide pneumoperitoneum.