Genome-Wide Association Study of Resistance to Largemouth Bass Ranavirus (LMBV) in Micropterus salmoides

• Published in: International journal of molecular sciences Vol. 25; no. 18; p. 10036
• Main Authors: Li, Pinhong, Luo, Xia, Zuo, Shaozhi, Fu, Xiaozhe, Lin, Qiang, Niu, Yinjie, Liang, Hongru, Ma, Baofu, Li, Ningqiu
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 18.09.2024; MDPI
• Subjects: Agricultural societies; Animals; Aquaculture; Bass; Bass - genetics; Bass - immunology; Bass - virology; Chromosomes; Disease; Disease Resistance - genetics; DNA Virus Infections - genetics; DNA Virus Infections - immunology; DNA Virus Infections - veterinary; DNA Virus Infections - virology; Expected values; Fish Diseases - genetics; Fish Diseases - immunology; Fish Diseases - virology; Fishes; Fresh water; Genes; Genetic aspects; Genetic markers; genome-wide association analyses (GWAS); Genome-Wide Association Study; Genomes; Genomics; Health aspects; Identification and classification; Immunity; INDEL Mutation; Infections; insertions–deletions (InDels); Largemouth bass; largemouth bass ranavirus (LMBV); Medical research; Medicine, Experimental; Micropterus salmoides; Mortality; Mutation; Physiological aspects; Polymorphism, Single Nucleotide; Proteins; Quality control; Ranavirus - physiology; Single nucleotide polymorphisms; single nucleotide polymorphisms (SNPs); Tilapia; Trout; China; Micropterus salmoides; insertions–deletions (InDels); largemouth bass ranavirus (LMBV); genome-wide association analyses (GWAS); single nucleotide polymorphisms (SNPs)
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms251810036

The disease caused by Largemouth bass ranavirus (LMBV) is one of the most severe viral diseases in largemouth bass ( ). It is crucial to evaluate the genetic resistance of largemouth bass to LMBV and develop markers for disease-resistance breeding. In this study, 100 individuals (45 resistant and 55 susceptible) were sequenced and evaluated for resistance to LMBV and a total of 2,579,770 variant sites (SNPs-single-nucleotide polymorphisms (SNPs) and insertions-deletions (InDels)) were identified. A total of 2348 SNPs-InDels and 1018 putative candidate genes associated with LMBV resistance were identified by genome-wide association analyses (GWAS). Furthermore, GO and KEGG analyses revealed that the 10 candidate genes ( , , , , , , , , , and ) were related to intestinal immune network for IgA production pathway and FoxO signaling pathway. The acquisition of candidate genes related to resistance will help to explore the molecular mechanism of resistance to LMBV in largemouth bass. The potential polymorphic markers identified in this study are important molecular markers for disease resistance breeding in largemouth bass.