Inflammatory alterations in excluded colon in rats: a comparison with chemically induced colitis

Diversion colitis occurs commonly in the large bowel remnant after diversion of the fecal stream. Several experimental models of colitis have been described, but none examine the inflammatory alterations that can occur in experimentally defunctioned colons. This characterization could be useful in u...

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Published inScandinavian journal of gastroenterology Vol. 45; no. 3; p. 315
Main Authors Longatti, Thamara Sigrist, Acedo, Simone Coghetto, de Oliveira, Caroline Candida, Miranda, Daniel Duarte da Conceição, Priolli, Denise Gonçalves, Ribeiro, Marcelo Lima, Gambero, Alessandra, Martinez, Carlos Augusto Real
Format Journal Article
LanguageEnglish
Published England 01.03.2010
Subjects
Animals
Colitis - chemically induced
Colitis - pathology
Colitis - physiopathology
Cyclooxygenase 2 - metabolism
Disease Models, Animal
Inflammation - physiopathology
Male
Nitric Oxide Synthase - metabolism
Rats
Rats, Wistar
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Summary:Diversion colitis occurs commonly in the large bowel remnant after diversion of the fecal stream. Several experimental models of colitis have been described, but none examine the inflammatory alterations that can occur in experimentally defunctioned colons. This characterization could be useful in understanding pathophysiological aspects of diversion colitis, and in developing future therapeutic strategies. Thus, we evaluated the temporal inflammatory alterations in the defunctioned colon of rats by analyzing the histological results, infiltrating neutrophils, pro-inflammatory markers such as cyclooxygenase (COX)-2 and inducible nitric oxide synthase (iNOS), and DNA damage in isolated colonocytes. We compared the obtained data with those from hapten-induced colitis. The experimental diversion of the colon fecal stream induces diversion colitis characterized by an early inflammatory process with increased neutrophil infiltrate, and COX-2 and iNOS expression that resembles, in some aspects, the inflammatory characteristics of chemically induced colitis. After acute inflammation resolution, there was an increase in COX-2 and iNOS expression and the presence of lymphoid follicular hyperplasia and ulcerations, suggesting that diversion colitis can be experimentally established and useful for studying different pathophysiological aspects of this condition.
ISSN:1502-7708
DOI:10.3109/00365520903471572