Virgibacillus xinjiangensis sp. nov., isolated from a Salt Lake of Xin-jiang Province in China

• Published in: The journal of microbiology Vol. 47; no. 6; pp. 705 - 709
• Main Authors: Jeon, Che Ok, Kim, Jeong Myeong, Park, Dong-Jin, Xu, Li-Hua, Jiang, Cheng-Lin, Kim, Chang-Jin
• Format: Journal Article
• Language: English
• Published: Heidelberg The Microbiological Society of Korea 01.12.2009; Springer Nature B.V; 한국미생물학회
• Subjects: Bacillaceae - chemistry; Bacillaceae - classification; Bacillaceae - genetics; Bacillaceae - isolation & purification; Base Composition; Biomedical and Life Sciences; Cell Wall - chemistry; China; Cluster Analysis; Deoxyribonucleic acid; Diaminopimelic Acid - analysis; DNA; DNA, Bacterial - chemistry; DNA, Bacterial - genetics; DNA, Ribosomal - chemistry; DNA, Ribosomal - genetics; Fatty Acids - analysis; Life Sciences; Microbiology; Molecular Sequence Data; Peptidoglycan - analysis; Phospholipids - analysis; Phylogeny; Quinones - analysis; RNA, Ribosomal, 16S - genetics; Salt lakes; Sequence Analysis, DNA; Sodium chloride; Water Microbiology; 생물학; China; sp. nov; Gram-positive; halotolerant
• ISSN: 1225-8873; 1976-3794; 1976-3794
• DOI: 10.1007/s12275-009-0107-9

A strictly aerobic Gram-positive, moderately halophilic spore forming bacterium, designated strain SL6-1 T , was isolated from a salt lake in Xin-jiang province, China. Growth of strain SL6-1 T was observed at NaCl concentrations of 0∼20% (w/v) (the optimum being 5∼7%, w/v). The peptidoglycan type of strain SL6-1 T was Alγ-meso-diaminopimelic acid and its major cellular fatty acids were iso-C 14:0 and iso-C 16:0 and ante-iso-C 15:0 . The major respiratory isoprenoid quinone was MK-7 and the G+C content of the genomic DNA was 44.5 mol%. The major cellular phospholipids were phosphatidylglycerol and diphosphatidylglycerol. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain SL6-1 T formed a phylogenetic lineage within the genus Virgibacillus . Based on 16S rRNA gene sequence similarity, the strain was most closely related to Virgibacillus olivae E 30 8 T , Virgibacillus kekensis YIM kkny16 T , Virgibacillus marismortui DSM 12325 T with 97.1%, 97.1%, and 97.0% gene sequence similarities, respectively and the sequence similarities to other related taxa were less than 96.7%. The DNA relatedness values between strain SL6-1 T and V. olivae E 30 8 T , V. kekensis YIM kkny16 T , V. marismortui DSM 12325 T were 16.7%, 51.0%, and 22.8%, respectively. On the basis of physiological, biochemical and phylogenetic properties, strain SL6-1 T represents a novel species, for which the name Virgibacillus xinjiangensis sp. nov. is proposed. The type strain is SL6-1 T (=KCTC 13128 T =DSM 19031 T ).