Hepatic necroinflammation and fibrosis in patients with genotypes Ba and C, core-promoter and precore mutations

• Published in: Journal of viral hepatitis Vol. 12; no. 5; pp. 513 - 518
• Main Authors: Yuen, M.-F., Tanaka, Y., Ng, I. O.-L., Mizokami, M., Yuen, J. C.-H., Wong, D. K.-H., Yuan, H.-J., Sum, S.-M., Chan, A. O.-O., Lai, C.-L.
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Science Ltd 01.09.2005
• Subjects: core-promoter mutation; fibrosis; Genotype; Hepatitis B Core Antigens - genetics; hepatitis B genotype; Hepatitis B virus - genetics; Hepatitis B, Chronic - pathology; Hepatitis B, Chronic - physiopathology; histology; Humans; Liver Cirrhosis - pathology; Liver Cirrhosis - physiopathology; Mutation; necroinflammation; precore mutation; Promoter Regions, Genetic
• ISSN: 1352-0504; 1365-2893
• DOI: 10.1111/j.1365-2893.2005.00629.x

The role of infection with hepatitis B virus (HBV) genotypes on liver histology is largely unknown. The aim of study was to investigate the relationships between HBV genotypes (B, C), core‐promoter (CP) and precore mutants and liver histology in 66 patients. Liver biopsies were scored by histologic activity index (HAI). HBV genotypes were determined by enzyme‐linked immunosorbent assay (ELISA). Eighteen (27.3%) and 48 patients (72.7%) had genotype B (all were subtype Ba) and C, respectively. Forty‐seven (71.2%) and 27 (40.9%) had CP and precore mutations, respectively. Patients with genotype C compared with subtype Ba had higher median scores of HAI‐necroinflammation (HAI‐NI) (7 vs 3), HAI‐fibrosis (HAI‐F) (1 vs 0) and total HAI (8.5 vs 3) (all P <  0.03). Patients with CP mutations compared with wild‐type had higher median scores of HAI‐NI (7 vs 3), HAI‐F (3 vs 0) and total HAI (9 vs 3) (all P <  0.03). Forty patients (83.5%) with genotype C had CP mutations. Age and alanine aminotransferase levels were positively correlated with HAI scores while albumin levels were negatively correlated (P < 0.01 for all, except albumin levels and HAI‐F, P = 0.08). There was no association between precore mutations and HAI scores. Multivariate analysis indicated that higher alanine aminotransferase (ALT) levels were associated with higher HAI scores (P < 0.04) and CP mutations were associated with higher HAI‐NI (P = 0.034), but not with HAI‐F score (P = 0.3). CP mutations were associated with more severe necroinflammation. The association between genotype C and poor histology was probably because of the association between genotype C and CP mutations.