Strategies for screening blood donors to source convalescent H1N1v plasma for intervention therapy
Background and Objectives During the 1918, pandemic blood components were successfully used to treat severe influenza pneumonia. A Proof of Principle trial investigating the clinical benefit of convalescent plasma was proposed in the 2009 H1N1v epidemic with the aim of screening donors for high tit...
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Published in | Vox sanguinis Vol. 103; no. 2; pp. 107 - 112 |
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Main Authors | , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Oxford, UK
Blackwell Publishing Ltd
01.08.2012
S. Karger AG |
Subjects | |
Online Access | Get full text |
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Summary: | Background and Objectives During the 1918, pandemic blood components were successfully used to treat severe influenza pneumonia. A Proof of Principle trial investigating the clinical benefit of convalescent plasma was proposed in the 2009 H1N1v epidemic with the aim of screening donors for high titre antibody in order to stockpile plasma packs to be used for treatment for severe pneumonia.
Materials and Methods Serum samples were collected from donors. IgG antibody capture format enzyme‐linked immunoassays using recombinant proteins (GACELISAs) were compared with microneutralization (MN) and haemagglutination inhibition (HAI). The influence of age and history of influenza‐like illness (ILI) on the detection of high titre antibody was examined.
Results 1598 unselected donor sera collected in October and December 2009 were tested by HAI. The HAI and demographic data defined a possible strategy for selective donor screening. One of the GACELISAs was highly specific for recent infection but showed lower sensitivity than HAI.
Conclusions During the 2009 pandemic screening 17‐ to 30‐year‐old donors by HAI delivered around 10% with high antibody levels. The ELISA using a short recombinant H1N1v HA detected fewer reactives but was more specific for high titre antibody (≥1:256). Screening strategies are proposed based on using HAI on serum or GACELISA on plasma. |
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Bibliography: | ArticleID:VOX1599 istex:9B8F83BC7C5834ACF2DE1ED6A9868C6D3299349F ark:/67375/WNG-X6KVL0XX-9 The work was carried out in the Virus Reference Department, Health Protection Agency Colindale, 61 Colindale Avenue, London NW9 5EQ. ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 |
ISSN: | 0042-9007 1423-0410 |
DOI: | 10.1111/j.1423-0410.2012.01599.x |