Catabolism of sulfamate by Mycobacterium sp. CF1
Summary A bacterium able to utilize sulfamate as N‐source for growth was isolated from soil and identified as a Mycobacterium sp. An apparently previously unrecorded enzyme, sulfamate hydrolase (EC 3.10.1.‐), converts sulfamate to equimolar amounts of ammonia and sulfate. This enzyme was purified to...
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Published in | Environmental microbiology Vol. 7; no. 3; pp. 378 - 381 |
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Main Authors | , |
Format | Journal Article |
Language | English |
Published |
Oxford, UK
Blackwell Science Ltd
01.03.2005
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Subjects | |
Online Access | Get full text |
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Summary: | Summary
A bacterium able to utilize sulfamate as N‐source for growth was isolated from soil and identified as a Mycobacterium sp. An apparently previously unrecorded enzyme, sulfamate hydrolase (EC 3.10.1.‐), converts sulfamate to equimolar amounts of ammonia and sulfate. This enzyme was purified to homogeneity and had a Km for sulfamate of 26.36 ± 4.01 mM. Its Specificity Constant value, 74 M−1 s−1, was low, indicating that it was not a particularly good catalyst for this reaction and it may be a hydrolase recruited to this role from some other reaction sequence. However, under equivalent conditions it showed no detectable action on the other sulfamates, cyclamate and sulfamoylbenzoate, or on urea or methylamine. |
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Bibliography: | ArticleID:EMI719 istex:ED16BC3326C654384C408D4D1F729DBC6CD2C26C ark:/67375/WNG-J3JHR154-8 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1462-2912 1462-2920 |
DOI: | 10.1111/j.1462-2920.2005.00719.x |