STR Melting Curve Analysis as a Genetic Screening Tool for Crime Scene Samples
: In this proof‐of‐concept study, high‐resolution melt curve (HRMC) analysis was investigated as a postquantification screening tool to discriminate human CSF1PO and THO1 genotypes amplified with mini‐STR primers in the presence of SYBR Green or LCGreen Plus dyes. A total of 12 CSF1PO and 11 HUMTHO...
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Published in | Journal of forensic sciences Vol. 57; no. 4; pp. 887 - 899 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
Oxford, UK
Blackwell Publishing Ltd
01.07.2012
Wiley Subscription Services, Inc |
Subjects | |
Online Access | Get full text |
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Summary: | : In this proof‐of‐concept study, high‐resolution melt curve (HRMC) analysis was investigated as a postquantification screening tool to discriminate human CSF1PO and THO1 genotypes amplified with mini‐STR primers in the presence of SYBR Green or LCGreen Plus dyes. A total of 12 CSF1PO and 11 HUMTHO1 genotypes were analyzed on the LightScanner HR96 and LS‐32 systems and were correctly differentiated based upon their respective melt profiles. Short STR amplicon melt curves were affected by repeat number, and single‐source and mixed DNA samples were additionally differentiated by the formation of heteroduplexes. Melting curves were shown to be unique and reproducible from DNA quantities ranging from 20 to 0.4 ng and distinguished identical from nonidentical genotypes from DNA derived from different biological fluids and compromised samples. Thus, a method is described which can assess both the quantity and the possible probative value of samples without full genotyping. |
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Bibliography: | ArticleID:JFO2106 istex:2C244D59931BE1B7BDC92C3DC4F058C46FF28F49 Presented in part at the Promega 17th International Symposium on Human Identification, October 10, 2006, in Nashville, TN. ark:/67375/WNG-JQC91LW3-7 Present address: VA Medical Center Center for Regenerative Medicine, C‐3113, 1481 West 10th Street, Indianapolis, IN 46202. ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0022-1198 1556-4029 |
DOI: | 10.1111/j.1556-4029.2012.02106.x |