Construction and characterisation of a Yersinia enterocolitica O:8 ompR mutant

• Published in: FEMS microbiology letters Vol. 165; no. 1; pp. 145 - 151
• Main Authors: Dorrell, Nick, Li, Shu-Rui, Everest, Pau H., Dougan, Gordon, Wren, Brendan W.
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.08.1998; Blackwell; Oxford University Press
• Subjects: Animals; Bacterial Proteins; Bacteriology; Base Sequence; Biological and medical sciences; Cloning, Molecular; Colony Count, Microbial; E coli; EnvZ protein; Fundamental and applied biological sciences. Psychology; Genes, Regulator; Genetics; High temperature; Homology; Hydrogen-Ion Concentration; Isogenic mutant; Mice; Mice, Inbred BALB C; Microbiology; Molecular Sequence Data; Mutants; Mutation; OmpR; Osmolar Concentration; Osmolarity; Pathogenesis; Polymerase Chain Reaction; Temperature; Trans-Activators - genetics; Trans-Activators - isolation & purification; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies; Virulence; Yersinia enterocolitica; Yersinia enterocolitica - genetics; Yersinia enterocolitica - pathogenicity; Yersiniosis; OmpR; Pathogenesis; Isogenic mutant; Characterization; Pathogenicity; Yersinia enterocolitica; Bacteria; Attenuation; Mutation; Molecular cloning; Attenuated strain; Enterobacteriaceae
• ISSN: 0378-1097; 1574-6968
• DOI: 10.1111/j.1574-6968.1998.tb13139.x

Abstract The ompR-envZ two-component regulatory system has been shown to contribute to virulence in a number of enteric bacterial pathogens. A Yersinia enterocolitica O:8 ompR homologue was amplified, cloned and sequenced, showing 99.2% homology to the Escherichia coli OmpR. An isogenic ompR mutant was constructed by reverse genetics-based methodology. The mutant was shown to have increased sensitivity to high osmolarity, high temperature and low pH stresses in vitro. In the murine yersiniosis model, the mutant was attenuated and offered partial protection against wild-type challenge.