Construction and characterisation of a Yersinia enterocolitica O:8 ompR mutant
Abstract The ompR-envZ two-component regulatory system has been shown to contribute to virulence in a number of enteric bacterial pathogens. A Yersinia enterocolitica O:8 ompR homologue was amplified, cloned and sequenced, showing 99.2% homology to the Escherichia coli OmpR. An isogenic ompR mutant...
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Published in | FEMS microbiology letters Vol. 165; no. 1; pp. 145 - 151 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
Oxford, UK
Blackwell Publishing Ltd
01.08.1998
Blackwell Oxford University Press |
Subjects | |
Online Access | Get full text |
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Summary: | Abstract
The ompR-envZ two-component regulatory system has been shown to contribute to virulence in a number of enteric bacterial pathogens. A Yersinia enterocolitica O:8 ompR homologue was amplified, cloned and sequenced, showing 99.2% homology to the Escherichia coli OmpR. An isogenic ompR mutant was constructed by reverse genetics-based methodology. The mutant was shown to have increased sensitivity to high osmolarity, high temperature and low pH stresses in vitro. In the murine yersiniosis model, the mutant was attenuated and offered partial protection against wild-type challenge. |
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Bibliography: | 1 Academic Department of Surgery, St. Bartholomew's and the Royal London School of Medicine and Dentistry, Whitechapel, London, UK. ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 |
ISSN: | 0378-1097 1574-6968 |
DOI: | 10.1111/j.1574-6968.1998.tb13139.x |