The SIMULTEST approach for testing mutagens in the Salmonella microtitre fluctuation assay

The concept of combining several histidine-dependent Salmonella strains in a single test, the SIMULTEST, has been applied to the microtitre fluctuation test. The activity of five mutagens was determined in strains TA97, TA98, TA100, and TA102 individually as well as in a SIMULTEST mixture. All five...

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Bibliographic Details
Published inEnvironmental and molecular mutagenesis Vol. 16; no. 1; p. 21
Main Authors McPherson, M F, Nestmann, E R
Format Journal Article
LanguageEnglish
Published United States 1990
Subjects
Culture Media
In Vitro Techniques
Mutagenicity Tests - methods
Mutagens - pharmacology
Salmonella typhimurium - drug effects
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Summary:The concept of combining several histidine-dependent Salmonella strains in a single test, the SIMULTEST, has been applied to the microtitre fluctuation test. The activity of five mutagens was determined in strains TA97, TA98, TA100, and TA102 individually as well as in a SIMULTEST mixture. All five compounds were mutagenic in the SIMULTEST, demonstrating the utility of this time and labour-saving approach of combining strains for testing with this method. The microtitre fluctuation SIMULTEST results were quantitatively comparable to those of the SIMULTEST Salmonella/microsome plate test. The microtitre fluctuation test compared with the plate incorporation assay generally showed more favourable "sensitivity" and "quantity" indices in that four of the five chemicals tested in the fluctuation test were mutagenic at lower doses than in the plate test.
ISSN:0893-6692
DOI:10.1002/em.2850160104