Production of N-acetylchitooligosaccharides by Aeromonas sp. DYU-TOO 7

• Published in: Journal of food biochemistry Vol. 29; no. 4; pp. 422 - 439
• Main Authors: Lien, T.S, Too, J.R, Wu, S.T, Yu, S.T
• Format: Journal Article
• Language: English
• Published: Oxford, UK and Malden, USA Blackwell Science Inc 01.08.2005; Blackwell
• Subjects: Aeromonas; Biological and medical sciences; biosynthesis; chitin; chitinase; culture filtrates; culture media; enzyme activity; Food industries; Fundamental and applied biological sciences. Psychology; hydrocolloids; microbial growth; oligosaccharides; protein content; serotypes; temperature; Bacteria; Vibrionaceae; Aeromonas; Production
• ISSN: 0145-8884; 1745-4514
• DOI: 10.1111/j.1745-4514.2005.00021.x

Aeromonas sp. DYU-Too 7 was screened to produce chitinases. The enzymes had an optimal reaction temperature of 50C and an optimal pH of 7.0. The best concentration of chitin in the fermentation process is 4%. Higher concentrations of chitin inhibited microbial growth and influenced the production of reducing sugars, the enzymatic activity and the protein content in the culture. N-acetylglucosamine, the main product in the hydrolysate, was 0.7 g/L at 36 h of cultivation. The concentration of N-acetylchitobiose was 0.16 g/L for the regular chitin broth and 0.28 g/L for the phosphorus-limiting chitin broth, with the highest concentration of N-acetylchitohexaose at about 0.062 g/L in both media. Experimental results showed that cultivation under a phosphorus-limiting condition substantially reduced the production of biomass, chitinases as well as N-acetylchitooligosaccharides.