DNA damage and repair induced by bleomycin in mammalian and insect cells

We assessed the response of mosquito (ATC-15) and mammalian (CHO) cells to bleomycin (BLM). Comparison of the data obtained in both cell lines indicates that DNA in the chromatin of mosquito cells is 10 to 20 times more resistant to BLM, and that the DNA damage induced by this antibiotic is better r...

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Bibliographic Details
Published inEnvironmental and molecular mutagenesis Vol. 17; no. 1; p. 63
Main Authors Bianchi, N O, López-Larraza, D M
Format Journal Article
LanguageEnglish
Published United States 1991
Subjects
Aedes
Animals
Bleomycin - pharmacology
Cell Division - drug effects
Cell Line
Cricetinae
Cricetulus
DNA Damage
DNA Repair
Dose-Response Relationship, Drug
Female
Kinetics
Mutagens
Ovary
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Summary:We assessed the response of mosquito (ATC-15) and mammalian (CHO) cells to bleomycin (BLM). Comparison of the data obtained in both cell lines indicates that DNA in the chromatin of mosquito cells is 10 to 20 times more resistant to BLM, and that the DNA damage induced by this antibiotic is better repaired in mosquito than in mammalian cells. Permeability of the cell membrane for BLM was found to be the same for both cell lines. Moreover, the time-kinetics of BLM damage to nuclear DNA was similar for ATC-15 and CHO cells. The low sensitivity of mosquito cells to BLM is reflected in better growth efficiency. These cells exhibit a satisfactory growth at BLM doses that produce a permanent arrest of growth in CHO cells. It is proposed that variations in the chromatin structure and in the intracellular free amino acid pool may play an important role in the differential response of insect and mammalian cells to BLM.
ISSN:0893-6692
DOI:10.1002/em.2850170110