IFN-mediated differential regulation of the expression of HLA-B7 and HLA-A3 class I genes

• Published in: The Journal of immunology (1950) Vol. 142; no. 1; pp. 297 - 305
• Main Authors: Hakem, R, Le Bouteiller, P, Barad, M, Trujillo, M, Mercier, P, Wietzerbin, J, Lemonnier, FA
• Format: Journal Article
• Language: English
• Published: Bethesda, MD Am Assoc Immnol 01.01.1989; American Association of Immunologists
• Subjects: Adjuvants, Immunologic - pharmacology; Animals; Biological and medical sciences; Cell Line, Transformed; DNA-Binding Proteins - metabolism; Fundamental and applied biological sciences. Psychology; Gene expression; Gene Expression Regulation - drug effects; Herpesvirus 4, Human; HLA-A Antigens - genetics; HLA-A3 Antigen; HLA-B Antigens - genetics; HLA-B7 Antigen; Humans; Interferon Type I - biosynthesis; Interferon Type I - genetics; Interferon Type I - pharmacology; Interferon-gamma - genetics; Interferon-gamma - pharmacology; L Cells (Cell Line); Lymphoma - genetics; Lymphoma - immunology; Mice; Molecular and cellular biology; Molecular genetics; Promoter Regions, Genetic - drug effects; T-Lymphocytes - immunology; Transcription, Genetic; Transfection; Human; Flow cytometry; Gel electrophoresis; Alpha interferon; Major histocompatibility system; Rodentia; Cytokine; Monoclonal antibody; HLA»-System; Gene expression; Biological activity; Vertebrata; Immunogenetics; Regulation(control); Mammalia; Transfection; Gene; Mouse; Beta interferon; T-Lymphocyte; Fibroblast; Class I histocompatibility antigen; Gamma interferon
• ISSN: 0022-1767; 1550-6606
• DOI: 10.4049/jimmunol.142.1.297

The regulation by IFN of the expression of HLA-B7 and HLA-A3 class I molecules was studied in Jurkat human T lymphoma cells, HHK EBV-transformed human B lymphocytes, and murine HLA-B7 HLA-A3 co-transfected L fibroblasts. Jurkat cells express constitutively low level of HLA class I molecules and treatment with human IFN resulted in preferential increase of the expression of HLA-B7 molecules, the expression of the HLA-A3 molecules being relatively unchanged. Similar treatment of HHK cells, which express constitutively large amount of HLA class I molecules, resulted in a marginal increase of the expression of both HLA-B7 and HLA-A3 molecules. HLA-B7 HLA-A3 co-transfected L cells express relatively low level of HLA class I molecules, expression of both however was significantly increased after treatment with murine INF-alpha, the augmentation being more accentuated for HLA-B7 molecules. In all cases, variations of cell surface expression were related to parallel modifications of the level of HLA-B7 and HLA-A3 RNA transcripts. Important nucleotide differences exist between the IFN consensus sequences associated with the HLA-B7 and HLA-A3 class I genes. Using oligonucleotides corresponding to these sequences two patterns of retarded bands were observed by the gel mobility shift assay, suggesting that the IFN-mediated differential regulation of the expression of the HLA-B7 and HLA-A3 genes could be due to different nuclear regulatory factors.