Selective target protein detection using a decorated nanopore into a microfluidic device

• Published in: Biosensors & bioelectronics Vol. 183; p. 113195
• Main Authors: Fujinami Tanimoto, Izadora Mayumi, Cressiot, Benjamin, Jarroux, Nathalie, Roman, Jean, Patriarche, Gilles, Le Pioufle, Bruno, Pelta, Juan, Bacri, Laurent
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 01.07.2021; Elsevier
• Subjects: Biosensing Techniques; Biotechnology; Chemical Sciences; Condensed Matter; Engineering Sciences; Lab-On-A-Chip Devices; Life Sciences; Micro and nanotechnologies; Microelectronics; Microfluidics; Nanopore transport; Nanopores; Nanotechnology; Physics; Polymer functionalization; Polymers; Protein sensing; Proteins; Soft Condensed Matter; Solid-state nanopore; Nanopore transport; Polymer functionalization; Protein sensing; Microfluidics; Solid-state nanopore
• ISSN: 0956-5663; 1873-4235
• DOI: 10.1016/j.bios.2021.113195

Solid-state nanopores provide a powerful tool to electrically analyze nanoparticles and biomolecules at single-molecule resolution. These biosensors need to have a controlled surface to provide information about the analyte. Specific detection remains limited due to nonspecific interactions between the molecules and the nanopore. Here, a polymer surface modification to passivate the membrane is performed. This functionalization improves nanopore stability and ionic conduction. Moreover, one can control the nanopore diameter and the specific interactions between protein and pore surface. The effect of ionic strength and pH are probed. Which enables control of the electroosmotic driving force and dynamics. Furthermore, a study of polymer chain structure and permeability in the pore are carried out. The nanopore chip is integrated into a microfluidic device to ease its handling. Finally, a discussion of an ionic conductance model through a permeable crown along the nanopore surface is elucidated. The proof of concept is demonstrated by the capture of free streptavidin by the biotins grafted into the nanopore. In the future, this approach could be used for virus diagnostic, nanoparticle or biomarker sensing. [Display omitted] •We control the pore diameter and specific interactions for a target biomolecule to increase the pore selectivity.•Each grafting step is followed at the nanometer scale by conductance measurements.•We detect the capture of specific target proteins using nanopore detection.•We control the dynamics through the nanopore according to the pH and the ionic force.