Rheological, biocompatibility and osteogenesis assessment of fish collagen scaffold for bone tissue engineering

• Published in: International journal of biological macromolecules Vol. 91; pp. 51 - 59
• Main Authors: Elango, Jeevithan, Zhang, Jingyi, Bao, Bin, Palaniyandi, Krishnamoorthy, Wang, Shujun, Wenhui, Wu, Robinson, Jeya Shakila
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.10.2016
• Subjects: Alkaline Phosphatase - metabolism; Animals; Biocompatibility; Biocompatible Materials - chemistry; Biocompatible Materials - metabolism; Biocompatible Materials - pharmacology; Biocompatible Materials - toxicity; Bone and Bones - cytology; Bone and Bones - drug effects; Bone and Bones - physiology; Cell Line, Tumor; Chitosan; Chitosan - chemistry; Collagen - chemistry; Collagen scaffold; Humans; Hydrogen-Ion Concentration; Hydroxyapatite; Mechanical Phenomena; Osteogenesis; Osteogenesis - drug effects; Porosity; Rheology; Sharks; Tissue Engineering; Tissue Scaffolds - chemistry; Viscosity; Water - chemistry; Biocompatibility; Hydroxyapatite; Chitosan; Collagen scaffold; Osteogenesis
• ISSN: 0141-8130; 1879-0003
• DOI: 10.1016/j.ijbiomac.2016.05.067

•Addition of chitosan had improved the mechanical properties of scaffold.•Higher level of ALP induced by collagen-hydroxyapatite scaffold.•Biodegradation rate of scaffold was increased by addition of chitosan. In the present investigation, an attempt was made to find an alternative to mammalian collagen with better osteogenesis ability. Three types of collagen scaffolds – collagen, collagen-chitosan (CCH), and collagen-hydroxyapatite (CHA) – were prepared from the cartilage of Blue shark and investigated for their physico-functional and mechanical properties in relation to biocompatibility and osteogenesis. CCH scaffold was superior with pH 4.5–4.9 and viscosity 9.7–10.9cP. Notably, addition of chitosan and HA (hydroxyapatite) improved the stiffness (11–23MPa) and degradation rate but lowered the water binding capacity and porosity of the scaffold. Interestingly, CCH scaffolds remained for 3days before complete in-vitro biodegradation. The decreased amount of viable T-cells and higher level of FAS/APO-1 were substantiated the biocompatibility properties of prepared collagen scaffolds. Osteogenesis study revealed that the addition of CH and HA in both fish and mammalian collagen scaffolds could efficiently promote osteoblast cell formation. The ALP activity was significantly high in CHA scaffold–treated osteoblast cells, which suggests an enhanced bone-healing process. Therefore, the present study concludes that the composite scaffolds prepared from fish collagen with higher stiffness, lower biodegradation rate, better biocompatible, and osteogenesis properties were suitable biomaterial for a bone tissue engineering application as an alternative to mammalian collagen scaffolds.