Protection of olive flounder, Paralichthys olivaceus, against viral hemorrhagic septicemia virus (VHSV) by immunization with NV gene-knockout recombinant VHSV

• Published in: Aquaculture Vol. 314; no. 1; pp. 39 - 43
• Main Authors: Kim, Min Sun, Kim, Ki Hong
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 04.04.2011; Amsterdam: Elsevier Science; Elsevier; Elsevier Sequoia S.A
• Subjects: Animal and plant ecology; Animal aquaculture; Animal productions; Animal, plant and microbial ecology; Aquaculture; Biological and medical sciences; fish; fish culture; fish farms; Flounder; Fundamental and applied biological sciences. Psychology; General aspects; Immunization; live vaccines; Marine; Mortality; neutralization; Olive flounder; Paralichthys olivaceus; Pleuronectiformes; rearing; Reverse genetics; rVHSV-ΔNV-EGFP; Sea water ecosystems; Synecology; vaccination; Vaccine potential; Vaccines; Viral hemorrhagic septicemia virus; virulence; Viruses; Vaccine potential; Olive flounder; Viral hemorrhagic septicemia virus; rVHSV-ΔNV-EGFP; Reverse genetics; Immunization; Vaccine; Virus; Marine environment; Mononegavirales; Vertebrata; Gene; Rhabdoviridae; Pisces; Genetics; Protection; Paralichthys olivaceus; Aquaculture
• ISSN: 0044-8486; 1873-5622
• DOI: 10.1016/j.aquaculture.2011.01.050

The potential of the NV gene-knockout recombinant viral hemorrhagic septicemia virus (rVHSV-ΔNV-EGFP) as a live attenuated vaccine was assessed. In an in vivo virulence experiment, olive flounder ( Paralichthys olivaceus) intramuscularly (i.m.) injected with 10 3 and 10 5 PFU of rVHSV-ΔNV-EGFP showed no mortality. The rVHSV-ΔNV-EGFP had ability to spread and replicate in the internal organs of olive flounder reared at either 15 °C or 20 °C. In the vaccination experiment, the cumulative mortality rates of olive flounder i.m. immunized with 10 3, 10 4, and 10 5 PFU of rVHSV-ΔNV-EGFP were 40–50%, 10–30%, and 0–10%, respectively, at 4 weeks after the primary immunization. In the second challenge experiment conducted at 4 weeks after the boost immunization, the cumulative mortality in fish immunized with 10 3 PFU of rVHSV-ΔNV-EGFP was 10–30%. No mortality was recorded in fish immunized with 10 4 and 10 5 PFU of the recombinant virus. While the cumulative mortality of fish in control group was 90–100%. The serum neutralization titers were also proportional to the survival rates of vaccine groups. The present results suggest that the rVHSV-ΔNV-EGFP has a high potential to be used as a live vaccine in olive flounder farms.