Rapid method for the determination of 14 isoflavones in food using UHPLC coupled to photo diode array detection

• Published in: Food chemistry Vol. 187; pp. 391 - 397
• Main Authors: Shim, You-Shin, Yoon, Won-Jin, Hwang, Jin-Bong, Park, Hyun-Jin, Seo, Dongwon, Ha, Jaeho
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 15.11.2015
• Subjects: Chromatography, High Pressure Liquid - methods; Food Analysis - methods; Glycine max - chemistry; Isoflavone; Isoflavones - analysis; Isoflavones - chemistry; Limit of Detection; Method validation; Rapid method; Reproducibility of Results; Soy Foods - analysis; Time Factors; UHPLC; Isoflavone; Rapid method; UHPLC; Method validation
• ISSN: 0308-8146; 1873-7072
• DOI: 10.1016/j.foodchem.2015.04.077

•Establishment of rapid and simple pretreatment method for 14 types of isoflavones.•Establishment of rapid detection method for 14 types of isoflavones using UHPLC/PDA.•Established method was validated through precision, accuracy and linearity.•Application test for various real samples such as beans and its processed products. A rapid method for the determination of 14 types of isoflavones in food using ultra-high performance liquid chromatography (UHPLC) was validated in terms of precision, accuracy, sensitivity and linearity. The UHPLC separation was performed on a reverse-phase C18 column (particle size 2μm, i.d. 2mm, length 100mm) using a photo diode array detector that was fixed to 260nm. The limits of detection and quantification of the UHPLC analyses ranged from 0.03 to 0.33mgkg−1. The intra-day and inter-day precision of the individual isoflavones were less than 11.77% and calibration curves exhibited good linearity (r2=0.99) within the tested ranges. These results suggest that the rapid method used in this study could be available to determine of 14 types of isoflavones in a variety of food such as soy bean, black bean, red bean and soybean paste.