Superparamagnetic iron oxide nanoparticles drive miR-485-5p inhibition in glioma stem cells by silencing Tie1 expression

• Published in: International journal of biological sciences Vol. 16; no. 7; pp. 1274 - 1287
• Main Authors: Pan, Zhiguang, Huang, Yongyi, Qian, Haiyang, Du, Xiling, Qin, Wenxing, Liu, Te
• Format: Journal Article
• Language: English
• Published: Australia Ivyspring International Publisher Pty Ltd 01.01.2020; Ivyspring International Publisher
• Subjects: 3' Untranslated regions; Angiogenesis; Approximation; Biotechnology; Brain-derived neurotrophic factor; Cancer; CD44 antigen; Chromosome 5; Efficiency; Electron clouds; Fibroblast growth factor 2; Gene expression; Glial cell line-derived neurotrophic factor; Glial fibrillary acidic protein; Glioma cells; Growth factors; In vivo methods and tests; Iron oxides; Kinases; Metastases; MicroRNAs; mRNA; Nanomaterials; Nanoparticles; Nervous system; Nucleic acids; Oct-4 protein; Research Paper; Signal transduction; Stem cells; Transmission electron microscopy; Tumors; Viability; United States > US; China; Superparamagnetic iron oxide nanoparticles (SPIONs); microRNA; glioma stem cells; Tie1
• ISSN: 1449-2288; 1449-2288
• DOI: 10.7150/ijbs.42887

Gliomas are highly malignant nervous system tumours. Studies shown that cancer stem cells are one of the main reasons underlying recurrence, metastasis, and poor prognosis in glioma cases. Our previous studies have found that superparamagnetic iron oxide nanoparticles (SPIONs) can act as nucleic acid carriers to drive intracellular overexpression of these nucleic acids. In this study, CD44+/CD133+ glioma stem cells (HuGSCs) were first isolated from surgically resected tissues from patients. qPCR and western blot results showed that Tie1 expression in HuGSCs was significantly higher thanexpression in CD44-/CD133- glioma cells. Bioinformatic analysis and luciferase reporter assays showed that miR-485-5p binds to specific loci on the 3'-UTR of mRNA to inhibit Tie1 expression. Subsequently, miR-485-5p/miR-mut and SPION complexes were transfected into HuGSCs. Transmission electron microscopy showed that a highly dense metallic electron cloud is present in HuGSCs. At the same time, and studies showed that miR-485-5p@SPIONs can significantly inhibit HuGSC proliferation, invasion, tumourigenicity, and angiogenesis. In-depth analysis showed that Tie1 interacts with neuronal growth factors such as FGF2, BDNF, GDNF, and GFAP. qPCR and western blot results showed that in miR-485-5p@SPIONs-HuGSCs, the expression levels of Tie1 and stem cell markers (Oct4, Sox2, Nanog, CD44, and CD133), and even FGF2, BDNF, GDNF, and GFAP were significantly lower than thelevels in the control group (miR-mut@SPIONs-HuGSCs). Therefore, this study showedthat Tie1 is an important factor that maintains glioma stem cell activity. SPIONs drive miR-485-5p overexpression in cells and inhibit endogenous Tie1 expression to downregulate the protein expression levels of Fgf2/GDNF/GFAP/BDNF and significantly weaken the and viability of gliomas.