The Role of Keratinocyte-derived Chemokine in Hemorrhage-induced Acute Lung Injury in Mice

• Published in: Journal of Korean medical science Vol. 24; no. 5; pp. 775 - 781
• Main Authors: Lee, Byoung Hoon, Lee, Tae Jin, Jung, Jae Woo, Oh, Dong Jin, Choi, Jae Chol, Shin, Jong Wook, Park, In Won, Choi, Byoung Whui, Kim, Jae Yeol
• Format: Journal Article
• Language: English
• Published: Korea (South) The Korean Academy of Medical Sciences 01.10.2009; 대한의학회
• Subjects: Acute Lung Injury - etiology; Acute Lung Injury - metabolism; Animals; Antibodies - immunology; Antibodies - metabolism; Chemokine CXCL2 - analysis; Chemokines - analysis; Chemokines - blood; Chemokines - physiology; Chickens; HMGB1 Protein - metabolism; Humans; Interleukin-1beta - analysis; Lipopolysaccharides - toxicity; Mice; Mice, Inbred BALB C; Neutrophils - immunology; Neutrophils - metabolism; NF-kappa B - metabolism; Original; Peroxidase - analysis; Shock, Hemorrhagic - complications; Time Factors; Tumor Necrosis Factor-alpha - analysis; 의학일반; Keratinocyte-derived Chemokines; Hemorrhage; Acute Lung Injury; LPS
• ISSN: 1011-8934; 1598-6357; 1598-6357
• DOI: 10.3346/jkms.2009.24.5.775

Dominant inflammatory cytokines might be different depending on the underlying causes of acute lung injury (ALI). The role of kertinocyte-derived chemokine (KC), a potent chemoattractant for neutrophils, has not been clearly established in hemorrhage-induced ALI. In this study, lung injury and cytokine expression were evaluated in LPS- or hemorrhage-induced ALI models of BALB/c mice. The myeloperoxidase activities at 4 hr after hemorrhage and LPS-injection were 47.4+/-13.0 and 56.5+/-16.4 U/g, respectively. NF-kappaB activity peaked at 4 hr after hemorrhage, which was suppressed to the control level by anti-high mobility group B1 (HMGB1) antibody. Lung expressions of TNF-alpha, MIP-2, and IL-1beta were increased by LPS injection. However, there was only a minimal increase in IL-1beta and no expressions of TNF-alpha or MIP-2 in hemorrhage-induced ALI. In contrast, lung KC increased significantly at 4 hr after hemorrhage compared to control levels (83.1+/-12.3 vs. 14.2+/-1.6 pg/mL/mg by ELISA) (P<0.05). By immunohistochemical staining, lung neutrophils stained positive for KC. Increased KC was also observed in bronchoalveolar lavage fluid and plasma. KC plays an important role in hemorrhage-induced ALI.