Changes of calcium binding proteins, c-Fos and COX in hippocampal formation and cerebellum of Niemann–Pick, type C mouse

• Published in: Journal of chemical neuroanatomy Vol. 52; pp. 1 - 8
• Main Authors: Byun, Kyunghee, Kim, Daesik, Bayarsaikhan, Enkhjaigal, Oh, Jeehyun, Kim, Jisun, Kwak, Grace, Jeong, Goo-Bo, Jo, Seung-Mook, Lee, Bonghee
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.09.2013
• Subjects: Animals; Calcium binding proteins; Calcium-Binding Proteins - metabolism; Cerebellum; Cerebellum - metabolism; Cerebellum - pathology; Cyclooxygenase 1 - metabolism; Cyclooxygenase 2 - metabolism; Female; Hippocampus; Hippocampus - metabolism; Hippocampus - pathology; Male; Membrane Proteins - metabolism; Mice; Mice, Inbred BALB C; Mice, Knockout; Mice, Transgenic; Niemann-Pick Disease, Type C - metabolism; Niemann-Pick Disease, Type C - pathology; NPC knock-out mice; Proto-Oncogene Proteins c-fos - antagonists & inhibitors; Proto-Oncogene Proteins c-fos - biosynthesis; Proto-Oncogene Proteins c-fos - metabolism; NPC; PBS; Cerebellum; NPC knock-out mice; DG; COX-1/COX-2; GABA; HP; NADP; PCR; Hippocampus; Calcium binding proteins; phosphate-buffered saline; gamma aminobutyric acid; dentate gyrus; Niemann–Pick disease, type C; cyclooxygenase-1/2; nicotinic acid adenine dinucleotide phosphate; polymerase chain reaction
• ISSN: 0891-0618; 1873-6300
• DOI: 10.1016/j.jchemneu.2013.04.006

•Parvalbumin, COX-2 or c-Fos neurons decreased in hippocampus and cerebellum.•Calbindin D28k or calretinin neurons decreased in hippocampus and cerebellum.•The decreased immunoreactivities were observed in 4- and 8-week old NPC−/− mice. Niemann–Pick disease, type C (NPC) is an intractable disease that is accompanied by ataxia, dystonia, neurodegeneration, and dementia due to an NPC gene defect. Disruption of calcium homeostasis in neurons is important in patients with NPC. Thus, we used immunohistochemistry to assess the expression levels of calcium binding proteins (calbindin D28K, parvalbumin, and calretinin), c-Fos and cyclooxygenase-1,2 (COX-1,2) in the hippocampal formation and cerebellum of 4 and 8 week old NPC+/+, NPC+/−, and NPC−/− mice. General expression of these proteins decreased in the hippocampus and cerebellum of NPC−/− compared to that in both young and adult NPC+/+ or NPC+/− mice. Parvalbumin, COX-1,2 or c-Fos-immunoreactive neurons were widely detected in the CA1, CA3, and DG of the hippocampus, but the immunoreactivities were decreased sharply in all areas of hippocampus of NPC−/− compared to NPC+/+ and NPC+/− mice. Taken together, reduction of these proteins may be one of the strong phenotypes related to the neuronal degeneration in NPC−/− mice.