Method of detecting β-lactam antibiotic induced vancomycin resistant MRSA (BIVR)

• Published in: International journal of antimicrobial agents Vol. 23; no. 1; pp. 1 - 5
• Main Authors: Hanaki, Hideaki, Yamaguchi, Yoshio, Nomura, Syuichi, Haraga, Isao, Nagayama, Ariaki, Sunakawa, Keisuke
• Format: Journal Article
• Language: English
• Published: London Elsevier B.V 2004; Amsterdam Elsevier; New York, NY
• Subjects: Antagonism; Antibacterial agents; Antibiotics. Antiinfectious agents. Antiparasitic agents; beta-Lactams - pharmacology; Biological and medical sciences; BIVR; Combination; Drug Antagonism; Humans; Medical sciences; Microbial Sensitivity Tests; MRSA; Pharmacology. Drug treatments; Staphylococcus aureus; Staphylococcus aureus - drug effects; Staphylococcus aureus - isolation & purification; Staphylococcus aureus - physiology; Vancomycin; Vancomycin - pharmacology; Vancomycin Resistance - physiology; β-Lactam antibiotic; Japan; Antagonism; Vancomycin; BIVR; β-Lactam antibiotic; Combination; MRSA; Peptides; Drug susceptibility test; Meticillin; Penicillin derivatives; Glycopeptide; Bacteria; Micrococcales; Micrococcaceae; Drug interaction; Clinical isolate; Staphylococcus aureus; Human; Drug combination; β-Lactams; In vitro; Resistance; Antibiotic; Sensitivity resistance; Antibacterial agent
• ISSN: 0924-8579; 1872-7913
• DOI: 10.1016/j.ijantimicag.2003.05.018

Despite the fact that the combination of vancomycin and a β-lactam antibiotic are known to act synergistically on vancomycin-susceptible Staphylococcus aureus (VSSA), some MRSA have emerged showing antagonism to the combination of vancomycin and a β-lactam antibiotic. These MRSA are called β-lactam antibiotic-induced vancomycin resistant MRSA (BIVR). A method based on this antagonistic phenomenon has been devised to detect BIVR strains. The method inhibits the VSSA strain but allows the BIVR strain to grow. Forty-six commercially available β-lactam antibiotics induced the vancomycin-resistance. Using this detection method, 717 MRSA clinical isolates obtained from eight institutes throughout Japan were thus screened and 6.3% of these were detected as BIVR when judged at 48 h.