Fast determination of biogenic amines in beverages by a core–shell particle column

• Published in: Food chemistry Vol. 187; pp. 555 - 562
• Main Authors: Preti, Raffaella, Antonelli, Marta Letizia, Bernacchia, Roberta, Vinci, Giuliana
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 15.11.2015
• Subjects: Biogenic amines; Biogenic Amines - analysis; Chromatography, High Pressure Liquid - instrumentation; Chromatography, High Pressure Liquid - methods; Core–shell particle column; Dansyl chloride; Food Contamination - analysis; Fruit nectar; HPLC; Wine; Wine - analysis; Wine; Putrescine (PubChem CID: 1045); Histamine (PubChem CID: 774); Core–shell particle column; Fruit nectar; Methylamine (PubChem CID: 6329); Tyramine (PubChem CID: 560); Dansyl chloride; Spermidine (PubChem CID: 1102); Ethylamine (PubChem CID: 6341); Agmatine (PubChem CID: 199); Cadaverine (PubChem CID: 273); Serotonin (PubChem CID: 5202); Biogenic amines; HPLC; Spermine (PubChem CID: 1103)
• ISSN: 0308-8146; 1873-7072
• DOI: 10.1016/j.foodchem.2015.04.075

•A fast HPLC determination of 11 biogenic amines in food has been described.•A new technology core–shell particle column has been used with UV and FLD detection.•This is the first article reporting the analysis of biogenic amines in fruit nectar.•The developed method resulted in a significant saving of analysis time and solvents. A fast and reliable HPLC method for the determination of 11 biogenic amines in beverages has been performed. After pre-column derivatization with dansyl-chloride a Kinetex C18 core–shell particle column (100mm×4.6mm, 2.6μm particle size) has been employed and the biogenic amines were identified and quantified in a total run time of 13min with ultraviolet (UV) or fluorescence detection (FLD). Chromatographic conditions such as column temperature (kept at 50°C), gradient elution and flow rate have been optimized and the method has been tested on red wine and fruit nectar. The proposed method is enhanced in terms of reduced analysis time and eluent consumption with respect of classical HPLC method as to be comparable to UHPLC methods. Green and cost-effective, this method can be used as a quality-control tool for routine quantitative analysis of biogenic amines in beverages for the average laboratory.