Chemical gelling of hydrogels-based biological macromolecules for tissue engineering: Photo- and enzymatic-crosslinking methods

• Published in: International journal of biological macromolecules Vol. 139; pp. 760 - 772
• Main Authors: Nezhad-Mokhtari, Parinaz, Ghorbani, Marjan, Roshangar, Leila, Soleimani Rad, Jafar
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 15.10.2019
• Subjects: Animals; Biocompatible Materials - chemistry; Catalysis; Cell Culture Techniques; Cell Survival; Chondrocytes - cytology; Collagen - chemistry; Cross-Linking Reagents - chemistry; Drug Combinations; Enzymatically-crosslinking; Enzymes - chemistry; Humans; Hydrogels; Hydrogels - administration & dosage; Hydrogels - chemistry; Injections; Laminin - chemistry; Mice; NIH 3T3 Cells; Osteoblasts - cytology; Photochemically cross-linking; Photochemistry - methods; Polymers - chemistry; Proteoglycans - chemistry; Tissue engineering; Tissue Engineering - methods; Hydrogels; Tissue engineering; Photochemically cross-linking; Enzymatically-crosslinking
• ISSN: 0141-8130; 1879-0003
• DOI: 10.1016/j.ijbiomac.2019.08.047

Newly, injectable hydrogels have been renowned as promising biomaterials and appropriate candidates for tissue engineering which can be applied for the development of 3-dimensional cell culture models. Hydrogels have in situ formability that allows an actual and homogeneous drugs/cells encapsulation, and suitable for in vivo surgical operation in a minimally invasive way, causing less discomfort for patients. A wide and varied range of methods has been applied to design hydrogels-based biological macromolecules via chemical gelling techniques, such as photo-polymerization, and enzyme-catalyzed reactions due to the biocompatibility and feasible processing of in situ formation of hydrogels and the easy implantation through in situ injection of hydrogels-based biological macromolecules. This present review covers the current advances in the development of injectable hydrogels through enzymatically and photo-crosslinking procedures for tissue engineering. The characteristics and applications of natural and synthetic base materials used in hydrogel generation are also reviewed with an outline on biomedical considerations. [Display omitted]