Inhibition of miR301 enhances Akt-mediated cell proliferation by accumulation of PTEN in nucleus and its effects on cell-cycle regulatory proteins

• Published in: Oncotarget Vol. 7; no. 15; pp. 20953 - 20965
• Main Authors: Jain, Mayur V, Shareef, Ahmad, Likus, Wirginia, Cieślar-Pobuda, Artur, Ghavami, Saeid, Łos, Marek J
• Format: Journal Article
• Language: English
• Published: United States Impact Journals LLC 12.04.2016
• Subjects: Apoptosis; Biomarkers, Tumor - metabolism; Breast Neoplasms - genetics; Breast Neoplasms - metabolism; Breast Neoplasms - pathology; Cell Cycle Proteins - metabolism; Cell Nucleus - metabolism; Cell Proliferation; Female; Humans; MicroRNAs - genetics; Phosphatidylinositol 3-Kinases - metabolism; Proto-Oncogene Proteins c-akt - metabolism; PTEN Phosphohydrolase - metabolism; Research Paper; Signal Transduction; Tumor Cells, Cultured; PTEN; mTOR; miR301; Akt; PI3K; AKT
• ISSN: 1949-2553; 1949-2553
• DOI: 10.18632/oncotarget.7996

Micro-RNAs (miRs) represent an innovative class of genes that act as regulators of gene expression. Recently, the aberrant expression of several miRs has been associated with different types of cancers. In this study, we show that miR301 inhibition influences PI3K-Akt pathway activity. Akt overexpression in MCF7 and MDAMB468 cells caused downregulation of miR301 expression. This effect was confirmed by co-transfection of miR301-modulators in the presence of Akt. Cells overexpressing miR301-inhibitor and Akt, exhibited increased migration and proliferation. Experimental results also confirmed PI3K, PTEN and FoxF2 as regulatory targets for miR301. Furthermore, Akt expression in conjunction with miR301-inhibitor increased nuclear accumulation of PTEN, thus preventing it from downregulating the PI3K-signalling. In summary, our data emphasize the importance of miR301 inhibition on PI3K-Akt pathway-mediated cellular functions. Hence, it opens new avenues for the development of new anti-cancer agents preferentially targeting PI3K-Akt pathway.