Accelerated Alveolar Bone Loss in Mice Lacking Interleukin-10

• Published in: Journal of dental research Vol. 82; no. 8; pp. 632 - 635
• Main Authors: Al-Rasheed, A., Scheerens, H., Rennick, D.M., Fletcher, H.M., Tatakis, D.N.
• Format: Journal Article
• Language: English
• Published: United States SAGE Publications 01.08.2003
• Subjects: Alveolar Bone Loss - classification; Alveolar Bone Loss - immunology; Alveolar Bone Loss - microbiology; Animals; Antibodies, Bacterial - blood; Bacteroides - immunology; Bacteroides fragilis - immunology; Dentistry; Immunoblotting; Interleukin-10 - blood; Interleukin-10 - immunology; Male; Mice; Mice, Inbred C57BL; Mice, Inbred Strains; Porphyromonas gingivalis - immunology; Prevotella intermedia - immunology; Statistics, Nonparametric; knockout; alveolar bone loss; antibodies; disease models; interleukin-10; mice
• ISSN: 0022-0345; 1544-0591
• DOI: 10.1177/154405910308200812

Interleukin-10 regulates pro-inflammatory cytokines, including those implicated in alveolar bone resorption. We hypothesized that lack of interleukin-10 leads to increased alveolar bone resorption. Male interleukin-10(−/−) mice, on 129/SvEv and C57BL/6J background, were compared with age-, sex-, and strain-matched interleukin-10(+/+) controls for alveolar bone loss. Immunoblotting was used for analysis of serum reactivity against bacteria associated with colitis and periodontitis. Interleukin-10(−/−) mice had significantly greater alveolar bone loss than interleukin-10(+/+) mice (p = 0.006). The 30–40% greater alveolar bone loss in interleukin-10(−/−) mice was evident in both strains, with C57BL/6J interleukin-10(−/−) mice exhibiting the most bone loss. Immunoblotting revealed distinct interleukin-10(−/−) serum reactivity against Bacteroides vulgatus, B. fragilis, Prevotella intermedia, and, to a lesser extent, against B. forsythus. The results of the present study suggest that lack of interleukin-10 leads to accelerated alveolar bone loss.