Cry toxin specificities of insect ABCC transporters closely related to lepidopteran ABCC2 transporters

• Published in: Peptides (New York, N.Y. : 1980) Vol. 98; pp. 86 - 92
• Main Authors: Endo, Haruka, Tanaka, Shiho, Imamura, Kazuhiro, Adegawa, Satomi, Kikuta, Shingo, Sato, Ryoichi
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.12.2017
• Subjects: ABC transporter subfamily C; Amino Acid Sequence; Animals; Bacillus thuringiensis; Bacterial Proteins - chemistry; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Bacterial Proteins - toxicity; Coleoptera - drug effects; Cry toxin; Diptera - drug effects; Endotoxins - chemistry; Endotoxins - metabolism; Endotoxins - toxicity; HEK293 Cells; Hemolysin Proteins - chemistry; Hemolysin Proteins - metabolism; Hemolysin Proteins - toxicity; Humans; Insect Proteins - chemistry; Insect Proteins - genetics; Insect Proteins - metabolism; Lepidoptera - drug effects; Multidrug Resistance-Associated Proteins - chemistry; Multidrug Resistance-Associated Proteins - genetics; Multidrug Resistance-Associated Proteins - metabolism; Phylogeny; Receptors, Cell Surface - chemistry; Receptors, Cell Surface - genetics; Receptors, Cell Surface - metabolism; ABCC transporter; HEK293T; LDH; PEI; ABC transporter subfamily C; Bacillus thuringiensis; ABC transporter; D-MEM; Cry toxin
• ISSN: 0196-9781; 1873-5169
• DOI: 10.1016/j.peptides.2017.04.003

•Moth ABCC2 and ABCC3 function as Cry1A toxin receptors.•A beetle ABCC transporter is a receptor of a beetle-specific Cry8C toxin.•Mosquito and human ABCC proteins do not respond to any of the tested Cry toxins. In this study, we examined insect and human ABCC transporters closely related to the lepidopteran ABC transporter C2 (ABCC2), a powerful receptor for the Bacillus thuringiensis Cry toxin, for their responses to various Cry toxins. ABCC2 and the lepidopteran ABC transporter C3 (ABCC3) conferred cultured cells with susceptibility to a lepidopteran-specific Cry1Aa toxin but not to lepidopteran-specific Cry1Ca and Cry1Da. One coleopteran ABCC transporter specifically responded to a coleopteran-specific Cry8Ca toxin. ABCC transporters from a dipteran insect and humans did not respond to any of the tested Cry toxins that are active to lepidopteran and coleopteran insects. These results yield important information for our understanding of insect specificity of Cry toxins and provide the first demonstration of a coleopteran ABCC transporter that serves as a Cry toxin receptor.