Differentiation of Campylobacter isolates on the basis of sensitivity to boiling in water as measured by PCR-detectable DNA

• Published in: Applied and environmental microbiology Vol. 64; no. 1; pp. 363 - 365
• Main Authors: MOHRAN, Z. S, ARTHUR, R. R, OYOFO, B. A, PERUSKI, L. F, WASFY, M. O, ISMAIL, T. F, MURPHY, J. R
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for Microbiology 1998
• Subjects: Bacteriological methods and techniques used in bacteriology; Bacteriology; Biological and medical sciences; Campylobacter coli - classification; Campylobacter coli - isolation & purification; Campylobacter coli - physiology; Campylobacter jejuni - classification; Campylobacter jejuni - isolation & purification; Campylobacter jejuni - physiology; DNA, Bacterial - analysis; DNA, Bacterial - isolation & purification; Egypt; Endopeptidase K - pharmacology; Fundamental and applied biological sciences. Psychology; Heating; Methods; Microbiology; Polymerase Chain Reaction - methods; Sodium Dodecyl Sulfate - pharmacology; Water; Africa; Egypt; Polymerase chain reaction; Sensitivity resistance; Campylobacteraceae; Campylobacter coli; Bacteria; Chromosome DNA; Isolate; Method; Differentiation; Boiling; Campylobacter jejuni
• ISSN: 0099-2240; 1098-5336
• DOI: 10.1128/aem.64.1.363-365.1998

Differential sensitivity for the release of PCR-detectable genomic DNA upon boiling in water is reported for 45 Campylobacter jejuni and Campylobacter coli strains isolated in Egypt. All of the strains released PCR-detectable DNA when treated with proteinase K and sodium dodecyl sulfate. When DNA was extracted from these strains by boiling in water, nine (20%) of the strains were PCR negative or resistant to boiling, suggesting the presence of boiling-sensitive and boiling-resistant phenotypes.