Hyperbaric oxygen therapy and ischemia and reperfusion: a valuable association to attenuate ischemic lesion and hepatic reperfusion

• Published in: Acta cirurgica brasileira Vol. 28; no. 2; pp. 126 - 130
• Main Authors: Losada, Daniele Moraes, Souza, Maria Eliza Jordani de, Jordani, Maria Cecília, Picinato, Maria Aparecida Neves Cardoso, Fina, Clarice Fleury, Feres, Omar, Michelone, Paulo Roberto Teixeira, Silva, Orlando de Castro e
• Format: Journal Article
• Language: English
• Published: Brazil Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia 01.02.2013
• Subjects: Alanine Transaminase - blood; Animals; Aspartate Aminotransferases - blood; Disease Models, Animal; Hyperbaric Oxygenation; Ischemia; Ischemia - pathology; Ischemia - therapy; Liver; Liver - blood supply; Liver - pathology; Male; Malondialdehyde - chemistry; Mitochondrial Swelling - physiology; Rats; Rats, Wistar; Reperfusion; Reperfusion Injury - therapy; Statistics, Nonparametric
• ISSN: 0102-8650; 1678-2674; 0102-8650
• DOI: 10.1590/S0102-86502013000200007

To investigate the consequences of the association between hyperbaric oxygen therapy and hepatic ischemia / reperfusion. Wistar rats were divided into three groups: SHAM, rats submitted to surgical stress and anesthetic but not hepatic ischemia or reperfusion, I / R, rats submitted to total hepatic pedicle ischemia for 30 min, followed by 5 min of reperfusion; HBO120, rats submitted to 120 min of hyperbaric oxygen therapy at two absolute atmospheres and immediately after submitted to the experimental protocol of ischemia and reperfusion. The preservation of the hepatic function was evaluated by determining mitochondrial swelling and malondialdehyde tissue level, as well as alanine aminotransferase and aspartate aminotranferase serum levels. The results were analyzed using the Mann-Whitney test and differences were considered significant for p<0.05. There were significant differences in values: mitochondrial swelling of the I / R group compared to SHAM and HBO120; malondialdehyde between SHAM vs. I / R, SHAM vs HBO120, and I / R vs HBO120, alanine aminotransferase between SHAM vs. I / R . There was no significant difference between groups in aspartate aminotransferase serum levels. The association between hyperbaric oxygen therapy and hepatic ischemia and reperfusion process was positive.