INHIBITION OF MALIGNANT MESOTHELIOMA CELL MATRIX METALLOPROTEINASE PRODUCTION AND INVASION BY A NOVEL NUTRIENT MIXTURE

• Published in: Experimental lung research Vol. 32; no. 3-4; pp. 69 - 79
• Main Authors: Roomi, M. Waheed, Ivanov, Vadim, Kalinovsky, Tatiana, Niedzwiecki, Aleksandra, Rath, Matthias
• Format: Journal Article
• Language: English
• Published: Philadelphia, PA Informa UK Ltd 01.03.2006; Taylor & Francis
• Subjects: ascorbic acid; Ascorbic Acid - pharmacology; Ascorbic Acid - therapeutic use; Biological and medical sciences; Camellia sinensis; Carcinogenesis, carcinogens and anticarcinogens; Cell Line, Tumor; Cell Proliferation - drug effects; Drug Combinations; Electrophoresis, Polyacrylamide Gel; Foods and miscellaneous; green tea extract; Humans; lysine; Lysine - pharmacology; Lysine - therapeutic use; malignant mesothelioma; matrigel invasion; Matrix Metalloproteinases - drug effects; Matrix Metalloproteinases - secretion; Medical sciences; Mesothelioma - drug therapy; Mesothelioma - pathology; MMPs; Neoplasm Invasiveness; nutrients; Plant Extracts - pharmacology; Plant Extracts - therapeutic use; Pneumology; Proline - pharmacology; Proline - therapeutic use; Tumors; Tumors of the respiratory system and mediastinum; Human; Ascorbic acid; Respiratory disease; Enzyme; Vitamin; MMPs; Proline; Metalloendopeptidases; Malignant tumor; Mixture; nutrients; Peptidases; Malignant mesothelioma; Lysine; green tea extract; Aminoacid; Plant origin; Hydrolases; Extracellular matrix; Nutrient; Tea extract; Inhibition; matrigel invasion; Green tea
• ISSN: 0190-2148; 1521-0499
• DOI: 10.1080/01902140600710488

Malignant mesothelioma (MM), an asbestos-associated cancer with no known cure, is a highly aggressive tumor causing profound morbidity and nearly universal mortality. Extracellular matrix (ECM) matrix metalloproteinases (MMPs) produced by tumor and stromal cells play a key role in tumor invasion and metastasis. Prevention of ECM degradation by MMP inhibition has been shown to be a promising therapeutic approach to inhibition of cancer development. Based on reported anticancer properties, the authors investigated the effect of a mixture (NM) containing lysine, proline, ascorbic acid, and green tea extract on MM cell line MSTO-211 H proliferation (by [MTT] [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] assay), MMP secretion (by gelatinase zymography), invasion (through Matrigel), and morphology (by hematoxylin and eosin [H&E] staining). MMP-2 and phorbol 12-myristate 13-acetate (PMA)-induced MMP-9 secretion were inhibited by NM in a dose-dependent fashion, with virtual total inhibition at 500 μg/ml NM. Invasion through Matrigel was inhibited at 50, 100, and 500 μg/ml by 27%, 36%, and 100%, respectively. NM was not toxic to the MM cell line, and H&E staining did not indicate any changes at and below 100 μg/ml concentration. In conclusion, NM significantly inhibited MM cell MMP secretion and invasion-both important parameters for cancer prevention, suggesting NM is an effective treatment strategy for MM.