Peyer's patch-immunomodulating glucans from sugar cane enhance protective immunity through stimulation of the hemopoietic system

• Published in: International journal of biological macromolecules Vol. 124; pp. 505 - 514
• Main Authors: Sakai, Yusuke, Sato, Masakazu, Funami, Yusuke, Ishiyama, Aki, Hokari, Rei, Iwatsuki, Masato, Nagai, Takayuki, Otoguro, Kazuhiko, Yamada, Haruki, Ōmura, Satoshi, Kiyohara, Hiroaki
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.03.2019
• Subjects: Glucans; Hemopoietic system; Malarial infection; Peyer's patches; Sugar cane; Hemopoietic system; Malarial infection; Peyer's patches; Sugar cane; Glucans
• ISSN: 0141-8130; 1879-0003
• DOI: 10.1016/j.ijbiomac.2018.11.180

The aim of the present study was chemical clarification of in vitro Peyer's patch-immunomodulating polysaccharides in sugar cane molasses, and evaluation of in vivo modulating activity on immune function of T lymphocytes in Peyer's patches and on microenvironment of hemopoietic system. Five kinds of glucans, comprising of dextranase-sensitive and activity-related d-glucosyl moieties, were purified as in vitro Peyer's patch-immunomodulating polysaccharides from the molasses. Oral administration of a glucan-enriched subfraction induced IL-2 and GM-CSF-producing T lymphocytes in Peyer's patches, resulting in enhancement of IL-6 production in a hemopoietic microenvironment to boost neutrophil numbers in the peripheral blood stream. Oral administration of purified glucan or glucan-enrich sub-fraction of sugar cane reduced the number of Plasmodium berghei- or P. yoelii-infected erythrocytes in a murine infection model, using polysaccharide alone or via co-administration with the antimalarial drug, artesunate. These results suggested that Peyer's patch-immunomodulating glucans enhanced protective immunity through axis of Peyer's patches-hemopoietic system. •Five kinds of in vitro Peyer’s patch-immunomodulating glucans were isolated from sugar cane molasses.•Oral administration of the active glucan subfraction stimulated IL-2 and GM-CSF productions from T lymphocytes in Peyer’s patches.•The active glucans increased peripheral granulocyte number by stimulation of IL-6 production in hemopoietic microenvironment.•The active glucans protected malarial infection by both glucan alone and combination with artesunate.