Identification and Validation of Hub Genes in the Stenosis of Arteriovenous Fistula

Arteriovenous fistula (AVF) is the most widely used hemodialysis vascular access in China. However, stenosis of the AVF limits its use. The mechanism of AVF stenosis is currently unknown. Therefore, the purpose of our study was to explore the mechanisms of AVF stenosis. In this study, we identified...

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Published inJournal of personalized medicine Vol. 13; no. 2; p. 207
Main Authors Li, Yu, Chen, Yue, Cui, Wenhao, Wang, Jukun, Chen, Xin, Zhang, Chao, Zhu, Linzhong, Bian, Chunjing, Luo, Tao
Format Journal Article
LanguageEnglish
Published Switzerland MDPI AG 25.01.2023
MDPI
Subjects
Activating transcription factor 3
Antibodies
Bioinformatics
Biomarkers
Carotid arteries
CXCR4 protein
Egr-2 protein
Fistula
Fistula, Arteriovenous
Fistulae
Gene expression
Genes
Genetic transcription
Hemodialysis
Hyperplasia
Laboratory animals
Medical research
Precision medicine
Proteins
Reverse transcription
Software
Stenosis
Therapeutic targets
Veins & arteries
China
Beijing China
Chicago Illinois
United States > US
hub gene
FOS
arteriovenous fistula
bioinformatic analysis
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Summary:Arteriovenous fistula (AVF) is the most widely used hemodialysis vascular access in China. However, stenosis of the AVF limits its use. The mechanism of AVF stenosis is currently unknown. Therefore, the purpose of our study was to explore the mechanisms of AVF stenosis. In this study, we identified the differentially expressed genes (DEGs) based on the Gene Expression Omnibus (GEO) dataset (GSE39488) between venous segments of AVF and normal veins. A protein-protein interaction (PPI) network was constructed to identify hub genes of AVF stenosis. Finally, six hub genes ( , , , , , and ) were found. Combined with the results of the PPI network analysis and literature search, and were selected as the target genes for further investigation. We validated the bioinformatic results via reverse transcription PCR (RT-PCR) and Western blot analyses on human and rat samples. The expression levels of the mRNA and protein of and were upregulated in both human and rat samples. In summary, we found that FOS may play an important role in AVF stenosis, which could be a potential therapeutic target of AVF stenosis.
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ISSN:2075-4426
2075-4426
DOI:10.3390/jpm13020207