Placentation and hormonal maintenance of pregnancy in the impala (Aepyceros melampus)

• Published in: Placenta (Eastbourne) Vol. 95; pp. 91 - 105
• Main Authors: Wilsher, Sandra, Greenwood, R.E.S., Mahon, G.D., Allen, W.R.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Ltd 01.06.2020
• Subjects: Antelope; Binucleate cells; Impala; Placenta; Placentome; Antelope; Placentome; Placenta; Impala; Binucleate cells
• ISSN: 0143-4004; 1532-3102
• DOI: 10.1016/j.placenta.2020.04.009

The impala is a widely distributed African ungulate. Detailed studies of the placenta and ovaries in impala undertaken in the 1970s did not address the endocrine functions of the placenta. The uteri of 25 pregnant impala estimated to be between 49 and 113 days of the 190 day gestation were examined grossly, histologically and immunohistochemically. A single corpus luteum was present in either maternal ovary but the conceptus was always situated in the right uterine horn. The fetal membranes extended to the tips of both uterine horns. The amnion was in intimate contact with, but not fused to, the allantochorion. Placentation was typically ruminant with fetal macrocotyledons attached to the rows of maternal caruncles. The fetal villi were highly branched, especially in the centre of each placentome where the attenuated maternal epithelium lining the placental crypts was absent in some places. Both the corpus luteum and the uninucleate trophoblast cells of the interplacentomal allantochorion stained strongly for 3-β hydroxysteroid dehydrogenase, and progestagen concentrations in allantoic and amniotic fluids increased significantly as gestation progressed, with a tendency to do likewise in maternal serum. Binucleate trophoblast cells stained positively for bovine placental lactogen, but neither the placenta nor the maternal corpus luteum showed evidence of oestrogen synthesis. Despite exhibiting the same basic type of placentation, both the gross and histological structure of the impala placenta, along with its immunohistochemical properties, demonstrates that great variation exists across ruminant placentas. •The fetus in its tubular amnion always occupies the larger right uterine horn.•The macrocotyledonary allantochorion extends to the tip of both uterine horns.•The placentomes of the impala are highly complex in their central regions.•Maternal crypts show erosion of their attenuated epithelial cells.•Trophoblast cells synthesise placental lactogen and progesterone but not oestrogens.