Characterization of monoclonal antibodies specific for the Lewis a human blood group determinant

• Published in: The Journal of biological chemistry Vol. 258; no. 8; pp. 4890 - 4894
• Main Authors: Young, W W, Johnson, H S, Tamura, Y, Karlsson, K A, Larson, G, Parker, J M, Khare, D P, Spohr, U, Baker, D A, Hindsgaul, O, Lemieux, R U
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 25.04.1983; American Society for Biochemistry and Molecular Biology
• Subjects: Animals; Antibodies, Monoclonal - immunology; Cell Line; Chromatography, Thin Layer; Humans; Infant, Newborn; Lewis Blood Group Antigens - immunology; Mice; Mice, Inbred BALB C; Rabbits
• ISSN: 0021-9258; 1083-351X
• DOI: 10.1016/S0021-9258(18)32510-9

Four hybridoma cell lines were derived from the spleen cells of mice immunized with the neutral glycolipids of human meconium. The antibodies secreted by these lines were specific for the Lewis a antigen of the human Lewis blood group system as determined by solid phase immunoassay using synthetic carbohydrate antigens and by plate binding assay and thin layer chromatography-autoradiography using natural glycolipid antigens. Coating protein A-bearing Staphylococcus aureus with one of the antibodies yielded a stable reagent that produced rapid agglutination of Lewis a positive human erythrocytes. The fine structural specificity of these antibodies was assessed by competition radioimmunoassay using synthetic structural analogs of Lewis a conjugated to bovine serum albumin. One antibody was specific for the Lewis a trisaccharide (Gal beta 1 leads to 3(Fuc alpha 1 leads to 4) beta GlcNAc), while a second recognized the entire Lea (1 leads to 3) beta Gal tetrasaccharide. The third and fourth were directed at topography largely provided by only the alpha Fuc and beta GlcNAc units. These monoclonal antibodies not only represent potentially useful reagents for detecting the Lewis a antigen but also provide a system for studying precise relationships between anticarbohydrate antibody structure and binding specificity.