Protection of cotton rats against human parainfluenza virus type 3 by vaccination with a chimeric FHN subunit glycoprotein

• Published in: Journal of general virology Vol. 74; no. 3; pp. 471 - 477
• Main Authors: Brideau, R. J, Oien, N. L, Lehman, D. J, Homa, F. L, Wathen, M. W
• Format: Journal Article
• Language: English
• Published: Reading Soc General Microbiol 01.03.1993; Society for General Microbiology
• Subjects: Animals; Antibodies, Viral - blood; Biological and medical sciences; Cell Division - immunology; Female; Fundamental and applied biological sciences. Psychology; Glycoproteins - immunology; Lymph Nodes - cytology; Male; Mice; Mice, Inbred BALB C; Microbiology; Parainfluenza Virus 3, Human - immunology; Recombinant Fusion Proteins - immunology; Sigmodontinae; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies; Vaccines, Synthetic - immunology; Viral Vaccines - immunology; Virology; Immunoprotection; Immune response; Rat; Antibody; Rodentia; Glycoproteins; Cellular immunity; Vaccine; Paramyxoviridae; Virus; Vertebrata; Mammalia; Immunogenicity; Paramyxovirus 3; Recombinant protein; Virus envelope; Lymphocyte; Paramyxovirus; Humoral immunity; Neutralization
• ISSN: 0022-1317; 1465-2099
• DOI: 10.1099/0022-1317-74-3-471

1 Cancer and Infectious Disease Research and 2 Molecular Biology Research, The Upjohn Company, 301 Henrietta Street, Kalamazoo, Michigan 49001, U.S.A. 49001 A cotton rat model of experimental human parainfluenza virus type 3 (PIV-3) infection was used to examine the efficacy of FHN, a novel chimeric glycoprotein which contains the extracellular regions of the fusion (F) and haemagglutinin—neuraminidase (HN) glycoproteins of PIV-3. The FHN protein was expressed in insect cells using a baculovirus vector system. FHN vaccination resulted in induction of neutralizing antibodies, was completely protective at doses of 100 ng, and was superior to vaccination with secreted forms F and HN proteins, or mixtures of the F and HN glycoproteins. In addition, FHN immunization induced lymphoproliferative responses in mice which were directed against both the F and HN glycoproteins. Fusion of the F and HN proteins into a single chimeric glycoprotein appeared to enhance the protective immune response compared to that elicited by the individual glycoproteins or mixtures of the two glycoproteins. Received 14 July 1992; accepted 19 October 1992.