Anti-fatigue activity of a novel polysaccharide conjugates from Ziyang green tea

• Published in: International journal of biological macromolecules Vol. 80; pp. 566 - 572
• Main Authors: Chi, Aiping, Li, Hong, Kang, Chenzhe, Guo, Huanhuan, Wang, Yimin, Guo, Fei, Tang, Liang
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.09.2015
• Subjects: Amino Acid Sequence; Animals; Exercise-induced fatigue; Fatigue - drug therapy; Fatigue - etiology; Male; Mice; Molecular Weight; Monosaccharides - chemistry; Physical Conditioning, Animal; Plant Extracts - chemistry; Plant Extracts - isolation & purification; Plant Extracts - pharmacology; Polysaccharide; Polysaccharides - chemistry; Polysaccharides - isolation & purification; Polysaccharides - pharmacology; Selenium; Tea - chemistry; Polysaccharide; Exercise-induced fatigue; Selenium
• ISSN: 0141-8130; 1879-0003
• DOI: 10.1016/j.ijbiomac.2015.06.055

•Polysaccharide from Ziyang green tea was a selenium-enriched polysaccharide conjugate (Se-TP).•Se-TP administrations significantly prolonged exhaustive time and increased glycogen level and GSH-Px activity in muscle.•Se-TP administrations markedly decreased BUN, Bla levels and LDH activity in serum and MDA level in muscle.•Se-TP treatments can significantly improve exercise-induced fatigue. The aim of this study was to investigate the anti-fatigue activity of polysaccharides from Ziyang green tea. Polysaccharides were isolated from Ziyang green tea and its physicochemical properties were analyzed. Meanwhile, a 4-week weight-loaded swimming test of mice was established and polysaccharides were orally administrated during exercise. The biochemical parameters related to fatigue were determined, such as exhaustive time, blood urea nitrogen (BUN), blood lactate acid (Bla) levels and lactic dehydrogenase (LDH) activity in serum, Superoxide dismutase (SOD), Glutathione peroxidase (GSH-Px) activities, Malondialdehyde (MDA) and glycogen levels in skeletal muscle. The results demonstrated that polysaccharide from Ziyang green tea was a selenium–polysaccharide–protein conjugate (Se-TP), and Se-TP administration significantly prolonged exhaustive time and increased glycogen level and GSH-Px activity in muscle, in addition, markedly decreased BUN, Bla levels and LDH activity in serum and MDA level in muscle. In conclusion, Se-TP treatment can significantly improve exercise-induced fatigue and decrease the oxidative stress induced by the exhaustive exercise.