Expression of Receptors for Advanced Glycosylation End Products on Renal Cell Carcinoma Cells in Vitro

Proteins that have been modified by long-term expose to glucose accumulate advanced glycosylation end products (AGEs) as a function of protein age. In these studies, we have examined the interaction of AGE-protein with renal cell carcinoma cells (RCC) in vitro, using AGE-modified bovine serum albumi...

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Published inBiochemical and biophysical research communications Vol. 196; no. 2; pp. 984 - 989
Main Authors Miki, S., Kasayama, S., Miki, Y., Nakamura, Y., Yamamoto, M., Sato, B., Kishimoto, T.
Format Journal Article
LanguageEnglish
Published San Diego, CA Elsevier Inc 29.10.1993
Elsevier
Subjects
Adenocarcinoma, Clear Cell - metabolism
Ageing, cell death
Base Sequence
Biological and medical sciences
Carcinoma, Renal Cell - metabolism
Cell Division
Cell physiology
Cells, Cultured
DNA Primers
Endothelium, Vascular - metabolism
Fundamental and applied biological sciences. Psychology
Gene Expression
Glycation End Products, Advanced - metabolism
Glycation End Products, Advanced - pharmacology
Humans
Interleukin-6 - biosynthesis
Molecular and cellular biology
Molecular Sequence Data
Polymerase Chain Reaction - methods
Receptor for Advanced Glycation End Products
Receptors, Immunologic - biosynthesis
RNA, Messenger - analysis
RNA, Messenger - biosynthesis
Tumor Cells, Cultured
Umbilical Veins
Urinary Bladder Neoplasms - metabolism
Human
Interleukin 6
Proteins
Molecular form
Cytokine
Molecular interaction
Glycosylation
Glucose
Age
Growth factor
Biological receptor
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Summary:Proteins that have been modified by long-term expose to glucose accumulate advanced glycosylation end products (AGEs) as a function of protein age. In these studies, we have examined the interaction of AGE-protein with renal cell carcinoma cells (RCC) in vitro, using AGE-modified bovine serum albumin (AGE-BSA) as a probe. AGE-BSA showed tendency to induce in vitro cell growth of RCC cells and promoted the production of interleukin-6 (IL-6), an in vitro autocrine growth factor. Reverse transcriptase-polymerase chain reaction analysis revealed that RCC cells used here express mRNA for a receptor for AGEs(RAGE). These results suggested that AGEs taken up through RAGE on RCC cells might play a role in promoting the growth of RCC cells.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.1993.2346