Extraction and physicochemical properties of polysaccharides from Ziziphus Jujuba cv. Muzao by ultrasound-assisted aqueous two-phase extraction

• Published in: International journal of biological macromolecules Vol. 108; pp. 541 - 549
• Main Authors: Ji, Xiaolong, Peng, Qiang, Yuan, Yuepeng, Liu, Fang, Wang, Min
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.03.2018
• Subjects: Antioxidants - chemistry; Antioxidants - isolation & purification; Antioxidants - pharmacology; Aqueous two-phase extraction; Extraction optimization; Free Radical Scavengers - chemistry; Free Radical Scavengers - isolation & purification; Free Radical Scavengers - pharmacology; Liquid-Liquid Extraction; Physicochemical property; Phytochemicals - chemistry; Polysaccharide; Polysaccharides - chemistry; Polysaccharides - isolation & purification; Polysaccharides - pharmacology; Solvents; Spectrophotometry, Ultraviolet; Spectroscopy, Fourier Transform Infrared; Temperature; Ziziphus - chemistry; Ziziphus Jujuba cv. Muzao fruit; PBS; UV; DPPH; BBD; Ziziphus Jujuba cv. Muzao fruit; ATPS; UAATPE; RSM; ATPE; FTIR; Physicochemical property; SEM; Extraction optimization; ZMP; Aqueous two-phase extraction; Polysaccharide; ABTS
• ISSN: 0141-8130; 1879-0003
• DOI: 10.1016/j.ijbiomac.2017.12.042

•UAATPE combined with ultrasound-aqueous two-phase extraction in one-step procedure.•Experimental conditions of UAATPE were optimized by RSM.•UAATPE conditions for jujube polysaccharides were firstly investigated.•Jujube polysaccharides were characterized by FTIR and derivative GC.•UAATPE was green, efficient and promising method to polysaccharide extraction. In this paper, ultrasound-assisted aqueous two-phase extraction (UAATPE) performed via a one-step procedure was applied to extract polysaccharides from Ziziphus Jujuba cv. Muzao. Using an ethanol/ammonium sulfate system as a multiphase solvent, effects of UAATPE on extraction of ZMP, such as solvent-to-material ratio, composition of aqueous two-phase system, and extraction time and temperature, were investigated using response surface methodology on basis of results of single-factor experiment. The final optimal conditions were ATPS composition: 29% ethanol and 15% (NH4)2SO4, extraction time: 38min, extraction temperature: 48°C, solvent-to-material ratio: 30, and microwave power: 70W. Under these conditions, the experimental extraction yield was 8.18%. Chemical analysis revealed that ZMP were composed of rhamnose, arabinose, xylose, mannose, glucose, galactose, and galacturonic acid, at a ratio of 1.46:2.47:2.27:1.12:1.00:1.57:5.40, respectively. The structures were also characterized with UV, FTIR, and SEM. The DPPH and ABTS radical scavenging activities showed ZMP had moderately antioxidant activity.