Hydrocarbon contamination affects deep-sea benthic oxygen uptake and microbial community composition

• Published in: Deep-sea research. Part I, Oceanographic research papers Vol. 100; pp. 79 - 87
• Main Authors: Main, C.E., Ruhl, H.A., Jones, D.O.B., Yool, A., Thornton, B., Mayor, D.J.
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 01.06.2015; Pergamon Press Inc
• Subjects: Bacteria; Benthic respiration; Blowout; Carbon; Communities; Contamination; Deep sea; Deep sea environments; Experimental incubations; Hydrocarbons; Isotopes; Marine; Microbiology; Microorganisms; Ocean bottom; Oceanography; Oxygen; Oxygen consumption; PLFA; Remineralisation; PLFA; Experimental incubations; Remineralisation; Benthic respiration; Blowout; Bacteria; Contamination
• ISSN: 0967-0637; 1879-0119
• DOI: 10.1016/j.dsr.2014.12.008

Accidental oil well blowouts have the potential to introduce large quantities of hydrocarbons into the deep sea and disperse toxic contaminants to midwater and seafloor areas over ocean-basin scales. Our ability to assess the environmental impacts of these events is currently impaired by our limited understanding of how resident communities are affected. This study examined how two treatment levels of a water accommodated fraction of crude oil affected the oxygen consumption rate of a natural, deep-sea benthic community. We also investigated the resident microbial community’s response to hydrocarbon contamination through quantification of phospholipid fatty acids (PLFAs) and their stable carbon isotope (δ13C) values. Sediment community oxygen consumption rates increased significantly in response to increasing levels of contamination in the overlying water of oil-treated microcosms, and bacterial biomass decreased significantly in the presence of oil. Multivariate ordination of PLFA compositional (mol%) data showed that the structure of the microbial community changed in response to hydrocarbon contamination. However, treatment effects on the δ13C values of individual PLFAs were not statistically significant. Our data demonstrate that deep-sea benthic microbes respond to hydrocarbon exposure within 36h. •A natural deep-sea sediment community was exposed to the water accommodated fraction of crude oil.•Hydrocarbon exposure increased sediment community oxygen consumption.•Microbial community structure (phospholipid fatty acid composition) changed significantly.•Hydrocarbons rapidly affect the structure and functioning of a deep-sea sediment community.