Influence of the peritoneal lavage with bupivacaine on the survival and resistance of colonic anastomoses performed under fecal peritonitis in rats

• Published in: Acta cirurgica brasileira Vol. 28; no. 11; pp. 783 - 787
• Main Authors: Camargo, Michel Gardere, Fagundes, João José, Leal, Raquel Franco, Ayrizono, Maria de Lourdes Setsuko, Rossi, Débora Helena dos Gonçalves, Oliveira, Priscilla de Sene Portel, Chung, Wu Feng, Lee, Huei Diana, Coy, Cláudio Saddy Rodrigues
• Format: Journal Article
• Language: English
• Published: Brazil Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia 01.11.2013
• Subjects: Anastomosis, Surgical; Anesthetics, Local; Anesthetics, Local - therapeutic use; Animals; Bupivacaine; Bupivacaine - therapeutic use; Colon; Colon - surgery; Disease Models, Animal; Feces; Male; Peritoneal Lavage - methods; Peritonitis; Peritonitis - mortality; Peritonitis - therapy; Postoperative Period; Random Allocation; Rats; Rats, Wistar; Reproducibility of Results; Survival Rate; Time Factors; Treatment Outcome
• ISSN: 0102-8650; 1678-2674; 0102-8650
• DOI: 10.1590/S0102-86502013001100007

To evaluate the effects of peritoneal lavage with bupivacaine on survival and initial resistance of anastomosis on distal colon, performed under peritonitis or not. Forty rats, weighing from 300 to 350 g (321.29 ± 11.3 1g), were randomly divided in four groups and underwent laparotomy and anastomosis on the distal colon six hours after induction of peritonitis by intraperitoneal injection of autologous fecal material or not. Group 1: No peritonitis and lavage with 3 ml NS; Group 2: No peritonitis and lavage with 8 mg.kg-1 (± 0.5 mL) of 0.5% bupivacaine added to 2.5 mL of NS; Group 3: Peritonitis and lavage with 3 ml NS; Group 4: Peritonitis and lavage with 8 mg.kg-1 (± 0.5 mL) of 0.5% bupivacaine added to 2.5 mL of NS. Necropsies were performed on the animals that died and the time of death was recorded. Surviving animals were submitted to euthanasia on the fifth post-operative day and Total Energy of Rupture biomechanical test (TER) was applied. Group 4 showed survival increase compared to Group 3, without statistical significance. Group 3 presented the smallest average TER, with statistical significance. Peritonitis increased mortality and influenced negatively the resistance of colic anastomosis in rats. Peritoneal lavage with bupivacaine increased anastomotic resistance.