Effect of fenugreek ( Trigonella foenum- graecum L.) galactomannan fractions on phagocytosis in rat macrophages and on proliferation and IgM secretion in HB4C5 cells

• Published in: Carbohydrate polymers Vol. 50; no. 1; pp. 79 - 83
• Main Authors: Ramesh, H.P., Yamaki, K., Tsushida, T.
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 01.10.2002; Elsevier Science
• Subjects: Activation analysis; Biological and medical sciences; Cell culture; Cells; Fenugreek galactomannan; General pharmacology; Growth kinetics; HB4C5 cells; IgM; Macrophages; Medical sciences; Molecular weight; Phagocytosis; Pharmacognosy. Homeopathy. Health food; Pharmacology. Drug treatments; Proliferation; Fenugreek galactomannan; HB4C5 cells; Proliferation; Macrophages; Phagocytosis; IgM; Human; Cell proliferation; Galactomannan; Rat; Pharmacognosy; Rodentia; Hybridoma; In vitro; Biological activity; Medicinal plant; Vertebrata; Mammalia; Leguminosae; Dicotyledones; Animal; Angiospermae; Established cell line; Plant origin; Spermatophyta; Macrophage; Peritoneum; Trigonella foenum graecum
• ISSN: 0144-8617; 1879-1344
• DOI: 10.1016/S0144-8617(01)00377-0

Fenugreek galactomannan rich fractions were tested for activation of phagocytosis in rat peritoneal macrophages, proliferation and IgM secretion in human–human hybridoma, HB4C5 cells. Alkali extracted polysaccharide B fraction showed 40% activation of phagocytosis in rat peritoneal macrophages at 10 μg/ml concentration. Fraction 1 of this extract showed an activity of 31.4% at 1 μg/ml in 1 h. In HB4C5 cells, growth promotion activities (%) expressed by alkali extracted polysaccharide B, Fraction 1 and Fraction 2 were 2.7 (at 5 μg/ml), 5.7 (at 2.5 μg/ml) and 5.6 (at 5 μg/ml), respectively. Effect on IgM secretion by alkali extracted polysaccharide B was more inhibitory with an inhibition of 24.0% at 2.5 μg/ml. Fraction 1 showed an activation of 9.8% at 5 μg/ml. At 2.5 μg/ml, it expressed an inhibition of 2.5%. Fraction 2 also expressed an inhibition of 24.7% at 2.5 μg/ml.