Influence of Conditions on Reuterin Accumulation by the Resting Cell Biotransformation Process

3-Hydroxypropionaldehyde （3-HPA） is a potential valuable chemical and new broad-spectrum antim-icrobial substance. In order to improve the conversion of 3-HPA/glycerol, our work studied the two-step process from glycerol to 3-HPA, and investigated the influence of cell harvest time, glycerol concent...

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Published inChinese journal of chemical engineering Vol. 19; no. 6; pp. 1023 - 1027
Main Author 陈国 杨道茂 肖雅琴 陈宏文
Format Journal Article
LanguageEnglish
Published Elsevier B.V 01.12.2011
Subjects
3-hydroxypropionaldehyde
Bioconversion
Biomass
Biotransformation
Chemical engineering
Conversion
Deactivation
Glycerols
Lactobacillus reuteri
reuterin
收获时间
甘油浓度
生物转化
生物量浓度
积累
转化条件
转化过程
静息细胞
reuterin
Lactobacillus reuteri
3-hydroxypropionaldehyde
bioconversion
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Summary:3-Hydroxypropionaldehyde （3-HPA） is a potential valuable chemical and new broad-spectrum antim-icrobial substance. In order to improve the conversion of 3-HPA/glycerol, our work studied the two-step process from glycerol to 3-HPA, and investigated the influence of cell harvest time, glycerol concentration, biomass con-centration, pH and temperature on the production of 3-HPA by Lactobacillus reuteri CG001, respectively. The re-sults showed that molar conversion yield of 3-HPA/glycerol reached 97.9% under the condition that 200 mmol·L-1 glycerol was converted by 25.3 g·L-1 resting cell for 1 h at 30 ℃. The cells could not be reused directly because the L. reuteri almost lost its bioconversion activity completely, but the ability of glycerol conversion could gradually recover if the fresh medium was added to the deactivated cell for 4 h.
Bibliography:Lactobacillus reuteri, 3-hydroxypropionaldehyde, reuterin, bioconversion
CHEN Guo, YANG Daomao , XIAO Yaqin , CHEN Hongwen Department of Bioengineering and Biotechnology, Huaqiao University, Xiamen 361021, China
3-Hydroxypropionaldehyde （3-HPA） is a potential valuable chemical and new broad-spectrum antim-icrobial substance. In order to improve the conversion of 3-HPA/glycerol, our work studied the two-step process from glycerol to 3-HPA, and investigated the influence of cell harvest time, glycerol concentration, biomass con-centration, pH and temperature on the production of 3-HPA by Lactobacillus reuteri CG001, respectively. The re-sults showed that molar conversion yield of 3-HPA/glycerol reached 97.9% under the condition that 200 mmol·L-1 glycerol was converted by 25.3 g·L-1 resting cell for 1 h at 30 ℃. The cells could not be reused directly because the L. reuteri almost lost its bioconversion activity completely, but the ability of glycerol conversion could gradually recover if the fresh medium was added to the deactivated cell for 4 h.
11-3270/TQ
ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ISSN:1004-9541
2210-321X
DOI:10.1016/s1004-9541(11)60086-4