Mesorhizobium alhagi sp. nov., isolated from wild Alhagi sparsifolia in north-western China

• Published in: International journal of systematic and evolutionary microbiology Vol. 60; no. Pt 4; pp. 958 - 962
• Main Authors: Chen, Wei-Min, Zhu, Wen-Fei, Bontemps, Cyril, Young, J Peter W, Wei, Ge Hong
• Format: Journal Article
• Language: English
• Published: England Microbiology Society 01.04.2010
• Subjects: Alphaproteobacteria - classification; Alphaproteobacteria - genetics; Alphaproteobacteria - isolation & purification; Alphaproteobacteria - physiology; Bacterial Proteins - genetics; Bacterial Typing Techniques; Bacteriology; Base Composition; Biodiversity; China; DNA, Ribosomal - analysis; DNA, Ribosomal - genetics; Fabaceae - microbiology; Fatty Acids - analysis; Genes, rRNA; Life Sciences; Microbiology and Parasitology; Molecular Sequence Data; Nucleic Acid Hybridization; Plant Roots - microbiology; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; Species Specificity; Symbiosis; Systematics, Phylogenetics and taxonomy; China
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/ijs.0.014043-0

Eleven strains that formed symbiotic root nodules on Alhagi sparsifolia, designated previously as genospecies II, were identified as a new lineage of Mesorhizobium (Alphaproteobacteria) that could be differentiated from all previously recognized species of the genus Mesorhizobium by using 16S rRNA gene sequences (<97.8 % similarity), DNA-DNA hybridization (<45 %), dnaJ, dnaK, recA, glnA, nifH, nodA and nodC gene sequences, fatty acid profiles (C(18 : 1)omega7c, 35 %;11-methyl C(18 : 1)omega7c, 30 %) and numerical taxonomy. These strains are therefore considered to represent a novel species, for which the name Mesorhizobium alhagi sp. nov. is proposed, with isolate CCNWXJ12-2(T) (=ACCC 15461(T)=HAMBI 3019(T)) as the type strain.