Spontaneous atopic dermatitis due to immune dysregulation in mice lacking Adamts2 and 14
Since its first description, ADAMTS14 has been considered as an aminoprocollagen peptidase based on its high similarity with ADAMTS3 and ADAMTS2. As its importance for procollagen processing was never experimentally demonstrated in vivo, we generated Adamts14-deficient mice. They are healthy, fertil...
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Published in | Matrix biology Vol. 70; pp. 140 - 157 |
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Main Authors | , , , , , , , , , , , , , |
Format | Journal Article Web Resource |
Language | English |
Published |
Netherlands
Elsevier B.V
01.09.2018
Elsevier Science Ltd Elsevier |
Subjects | |
Online Access | Get full text |
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Summary: | Since its first description, ADAMTS14 has been considered as an aminoprocollagen peptidase based on its high similarity with ADAMTS3 and ADAMTS2. As its importance for procollagen processing was never experimentally demonstrated in vivo, we generated Adamts14-deficient mice. They are healthy, fertile and display normal aminoprocollagen processing. They were further crossed with Adamts2-deficient mice to evaluate potential functional redundancies between these two highly related enzymes. Initial characterizations made on young Adamts2-Adamts14-deficient animals showed the same phenotype as that of Adamts2-deficient mice, with no further reduction of procollagen processing and no significant aggravation of the structural alterations of collagen fibrils. However, when evaluated at older age, Adamts2-Adamts14-deficient mice surprisingly displayed epidermal lesions, appearing in 2 month-old males and later in some females, and then worsening rapidly. Immunohistological evaluations of skin sections around the lesions revealed thickening of the epidermis, hypercellularity in the dermis and extensive infiltration by immune cells. Additional investigations, performed on young mice before the formation of the initial lesions, revealed that the primary cause of the phenotype was not related to alterations of the epidermal barrier but was rather the result of an abnormal activation and differentiation of T lymphocytes towards a Th1 profile. However, the primary molecular defect probably does not reside in the immune system itself since irradiated Adamts2-Adamts14-deficient mice grafted with WT immune cells still developed lesions.
While originally created to better characterize the common and specific functions of ADAMTS2 and ADAMTS14 in extracellular matrix and connective tissues homeostasis, the Adamts2-Adamts14-deficient mice revealed an unexpected but significant role of ADAMTS in the regulation of immune system, possibly through a cross-talk involving mesenchymal cells and the TGFβ pathways.
•Based on in vitro studies, ADAMTS14 might be an aminoprocollagen peptidase in vivo•We show that ADAMTS14 does not have aminoprocollagen peptidase activity in vivo•Mice deficient for both ADAMTS2 and ADAMTS14 spontaneously develop epidermal lesions•ADAMTS2 and ADAMTS14 participate in the regulation of the immune system•The absence of both ADAMTS2 and ADAMTS14 leads to an atopic dermatitis-like phenotype |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 scopus-id:2-s2.0-85045550492 |
ISSN: | 0945-053X 1569-1802 1569-1802 |
DOI: | 10.1016/j.matbio.2018.04.002 |