Reconstruction of abdominal wall defects using small intestinal submucosa coated with gelatin hydrogel incorporating basic fibroblast growth factor

• Published in: Acta cirurgica brasileira Vol. 29; no. 4; pp. 252 - 260
• Main Authors: Wang, Liang, Lai, Dong-ming, Yang, Bin, Jiang, Zhi-peng, Zhang, Yu-chao, Zhou, Jun, Lai, Wei, Chen, Shuang
• Format: Journal Article
• Language: English
• Published: Brazil Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia 01.04.2014
• Subjects: Abdominal Wall; Abdominal Wall - pathology; Abdominal Wall - surgery; Animals; Biocompatible Materials; Biocompatible Materials - therapeutic use; Collagen - analysis; Fibroblast Growth Factor 2 - therapeutic use; Gelatin - therapeutic use; Hydrogel, Polyethylene Glycol Dimethacrylate - therapeutic use; Immunohistochemistry; Intestinal Mucosa - transplantation; Intestinal, Fibroblast Growth Factor 2; Intestine, Small; Materials Testing; Random Allocation; Rats; Rats, Sprague-Dawley; Regeneration; Reproducibility of Results; Time Factors; Tissue Adhesions; Treatment Outcome
• ISSN: 0102-8650; 1678-2674; 0102-8650
• DOI: 10.1590/S0102-86502014000400006

To construct a new biomaterial-small intestinal submucosa coated with gelatin hydrogel incorporating basic fibroblast growth factor, and to evaluate the new biomaterials for the reconstruction of abdominal wall defects. Thirty six Sprague-Dawley rats were used in the animal experiments and randomly divided into three groups. The new biomaterial was constructed by combining small intestinal submucosa with gelatin hydrogel for basic fibroblast growth factor release. Abdominal wall defects were created in rats, and repaired using the new biomaterials (group B), compared with small intestinal submucosa (group S) and ULTRAPROTM mesh (group P). Six rats in each group were sacrificed at three and eight weeks postoperatively to examine the gross effects, inflammatory responses, collagen deposition and neovascularization. After implantation, mild adhesion was caused in groups B and S. Group B promoted more neovascularization than group S at three weeks after implantation, and induced significantly more amount of collagen deposition and better collagen organization than groups S and P at eight weeks after implantation. Small intestinal submucosa coated with gelatin hydrogel incorporating basic fibroblast growth factor could promote better regeneration and remodeling of host tissues for the reconstruction of abdominal wall defects.