Tryptophan-functionalized gold nanoparticles for deep UV imaging of microbial cells
•Gold nanoparticles were functionalized with amino acid tryptophan (Trp).•Trp layers on Au yielded photoluminescence of the nanostructures in the UV region.•Au–Trp nanostructures were used as fluorescent probes for DUV imaging of E. coli.•The spatial distribution of the nanoparticles within individu...
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Published in | Colloids and surfaces, B, Biointerfaces Vol. 135; pp. 742 - 750 |
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Main Authors | , , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Netherlands
Elsevier B.V
01.11.2015
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Subjects | |
Online Access | Get full text |
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Summary: | •Gold nanoparticles were functionalized with amino acid tryptophan (Trp).•Trp layers on Au yielded photoluminescence of the nanostructures in the UV region.•Au–Trp nanostructures were used as fluorescent probes for DUV imaging of E. coli.•The spatial distribution of the nanoparticles within individual cells was followed.
Deep UV fluorescence image of E. coli cells incubated with tryptophan-functionalized gold nanoparticles.
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Biocompatible fluorescent nanostructures were prepared by a functionalization of gold nanoparticles with the amino acid tryptophan. The gold-tryptophan bioconjugates were investigated by TEM and HRTEM and various spectroscopy methods (XPS, FTIR, UV–vis and photoluminescence). It was found that the gold nanoparticles, initially 8nm in diameter, aggregate in the presence of the amino acid. From the XPS and FTIR spectroscopy results, it was concluded that the tryptophan gold interactions mainly take place via indole and carboxyl groups. Although the indole group is involved in the interaction with the gold surfaces, the tryptophan–gold hybrids showed strong fluorescence due to the presence of multilayers of tryptophan. Deep ultra violet (DUV) imaging performed at the SOLEIL synchrotron showed that it is possible to detect these hybrid nanostructures within Escherichia coli cells. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0927-7765 1873-4367 |
DOI: | 10.1016/j.colsurfb.2015.08.050 |