Reciprocal Regulation by Cyclic Nucleotides of the Differentiation of Rat Osteoblast-like Cells and Mineralization of Nodules

The effects of cAMP and cGMP on the differentiation of osteoblast-like cells derived from rat calvariae and on the formation of bone in vitro were studied. Continuous culture of osteoblast-like cells in the presence of 8-bromo-cyclic AMP (8-Br-cAMP) resulted in the dose-related inhibition both of th...

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Published inBiochemical and biophysical research communications Vol. 215; no. 3; pp. 1104 - 1110
Main Authors Inoue, A., Hiruma, Y., Hirose, S., Yamaguchi, A., Hagiwara, H.
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 24.10.1995
Subjects
8-Bromo Cyclic Adenosine Monophosphate - pharmacology
Alkaline Phosphatase - antagonists & inhibitors
Alkaline Phosphatase - biosynthesis
Animals
Animals, Newborn
Biomarkers
Blotting, Northern
Bone Development - drug effects
Bone Development - physiology
Cell Differentiation
Cyclic AMP - metabolism
Cyclic GMP - analogs & derivatives
Cyclic GMP - metabolism
Cyclic GMP - pharmacology
Homeostasis
Kinetics
Osteoblasts - cytology
Osteoblasts - drug effects
Osteoblasts - physiology
Rats
Rats, Sprague-Dawley
RNA, Messenger - analysis
RNA, Messenger - biosynthesis
Skull - cytology
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Summary:The effects of cAMP and cGMP on the differentiation of osteoblast-like cells derived from rat calvariae and on the formation of bone in vitro were studied. Continuous culture of osteoblast-like cells in the presence of 8-bromo-cyclic AMP (8-Br-cAMP) resulted in the dose-related inhibition both of the synthesis of cellular alkaline phosphatase (ALPase), which is known as a marker of osteoblastic differentiation, and of the formation of mineralized nodules, which is a model of the formation of bone in vitro. By contrast, 8-bromo-cyclic GMP (8-Br-cGMP) promoted the synthesis of ALPase and the formation of mineralized nodules. Northern blot analysis revealed that these cyclic nucleotides modulated the steady-state levels of mRNAs for ALPase and osteocalcin, a bone-matrix protein that is specifically produced by osteoblast. The present results indicate that cAMP and cGMP act reciprocally to regulate osteoblastic differentiation and the subsequent formation of mineralized nodules.
Bibliography:ObjectType-Article-1
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ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.1995.2577