Ethanol production from inulin and unsterilized meal of Jerusalem artichoke tubers by Saccharomyces sp. W0 expressing the endo-inulinase gene from Arthrobacter sp

• Published in: Bioresource technology Vol. 147; pp. 254 - 259
• Main Authors: Li, Yang, Liu, Guang-Lei, Chi, Zhen-Ming
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.11.2013
• Subjects: Arthrobacter; Arthrobacter - genetics; Base Sequence; DNA Primers; Endo-inulinase; Ethanol; Ethanol - metabolism; Ethyl alcohol; Fermentation; Genes; Glycoside Hydrolases - genetics; Glycoside Hydrolases - metabolism; Helianthus - metabolism; Inulin; Inulin - metabolism; Inulinase; Jerusalem artichoke; Recombinant; RNA, Ribosomal, 18S - genetics; Saccharomyces; Saccharomyces - metabolism; Saccharomyces sp. W0; Yeast; Ethanol; Endo-inulinase; Jerusalem artichoke; Saccharomyces sp. W0; Inulin
• ISSN: 0960-8524; 1873-2976
• DOI: 10.1016/j.biortech.2013.08.043

•The endo-inulinase gene was expressed Saccharomyces sp. W0.•Inulinase and ethanol production in the recombinant yeast were enhanced.•The unsterilized meal of Jerusalem artichoke tubers was converted into ethanol. After the endo-inulinase gene from Arthrobacter sp. was ligated the expression vectors pMIDSC31 and pMIRSC31, the endo-inulinase gene was inserted into the chromosomal DNA of Saccharomyces sp. W0. It was found that the inulinase activity of the recombinant yeast D5 in which the endo-inulinase gene was inserted into the delta sequence was higher than that of the recombinant yeast R1 in which the endo-inulinase gene was inserted into 18S rDNA sequence. More ethanol from inulin was produced by the recombinant yeast D5 than by the recombinant yeast R1. But Saccharomyces sp. W0 produced the lowest inulinase activity and concentration of ethanol. During the 3-l fermentation, the recombinant yeast D5 could produce 13.6ml of ethanol per 100ml of the fermented medium from 30% inulin. The recombinant yeast D5 could actively convert the unsterilized meal of Jerusalem artichoke tubers, yielding 10.1ml of ethanol per 100ml of the fermented medium.